Analysis of Immune Memory Dynamics by visualizing memory B cell subsets

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We have established a new mouse line in which activated IgM+ B cells are specifically labeled and can be inducibly depleted. The M-SDV mice have loxp-flanked STOP cassette followed by DTR-Venus expression construct in Ig heavy chain Cmu locus. If Cre-mediated recombination occurs, IgM+ B cells should express DTR-Venus fusion protein. We crossed M-SDV line with AID-cre. After immunization, a fraction of IgM+ B cells became DTR-Venus positive, while IgM- B cells remained DTR-Venus negative. Further, when the mice were administered with DT, the DTR-Venus positive cells were completely deleted. These results suggest that M-SDV mouse model is an excellent system to detect or deplete IgM memory B cells whose function is largely unknown.