Development of drugs targeting hepatitis B x-protein

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We have conducted study project to develop new classes of antiviral drug against hepatitis B virus (HBV). In the study period, we obtained the following results. (1) HBV expression plasmid which selectively lacks HBX protein showed decreased expression levels as compared with wild type when transfected into humans hepatoma Huh7 cells. (2) Mutant HBV expression plasmids were constructed in which 4 consecutive alanine substitution were introduced in the HBX protein coding frame. By using the mutant HBX plasmids, we found AP1 transactivator domain in C-terminal of HBX.