Development of methods to define the pathogenic magnitude of the influenza virus infection using human airway epithelial cells

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In the present study using human airway epithelial cells infected with four strains of influenza viruses, increases in the viral release and interleukin (IL)-6, which are associated with the pathogenic magnitude of the influenza virus infection, were correlated to the number of detached cells and the concentration of lactate dehydrogenase (LDH) in the supernatants. Because these parameters are associated with the pathogenesis of the patients, the experimental methods to infect airway epithelial cells with influenza virus may be useful to define the pathogenic magnitude of the influenza virus.The consistent replication of influenza virus in the airway epithelial cells was also detected at 39.0 degrees C, but the increased concentrations of LDH in the supernatants were observed. Appropriate use of anti-influenza virus drugs and improvement of cell functions by normalizing body temperature with anti-inflammatory drugs are needed to prevent severe conditions by influenza virus infection.