A novel function of Interferon regulatory factor-1

我们重点关注IRF-1在病原体感染过程中的作用，特别是IRF-1在病原体感染过程中对Th 2抑制的参与。我们发现IRF-1直接抑制Th 2细胞IL-4基因的转录。IRF-1在李斯特菌感染的APC产生的可溶性因子的作用下与IL-4基因沉默区结合，下调IL-4基因的转录。我们确定IL-1介导可溶性因子的活性。根据所呈现的结果，我们提出IRF-1的功能通过两种不同的机制诱导Th 1移位，先前已知的机制和这里描述的新功能。IRF-1激活IL-12 p40基因启动子活性，诱导基因转录，使IL-12向Th 1亚群转移。此外，IRF-1通过IL-1的影响作用于IL-4基因的沉默区，并抑制IL-4基因在Th 2细胞中的转录。

We focused on the role of IRF-1 during a pathogen infection, especially the involvement of IRF-1 in the suppression of Th2 during the pathogen infection. We found that IRF-1 directly suppresses the transcription of Il4 gene of Th2 cells. IRF-1 binds to the silencer region of Il4 gene by the influence of soluble factor that produced by Listeria-infected APCs and down-regulates the Il4 gene transcription. We identified that IL-1 mediates the activity of the soluble factor. According to the results presented, we propose that IRF-1 functions to induce the Th1 shift via two distinct mechanisms, the previously known mechanism and a novel function described here. IRF-1 activates the Il12 p40 gene promoter activity and induces the gene transcription, resulted in the shift to Th1 subset. In addition, IRF-1 acts on the silencer region of Il4 gene by the influence of IL-1 and inhibits the transcription of the Il4 gene in Th2 cells.