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Linkage between innate immunity and adaptive immunity during pahogen infection

病原体感染期间先天免疫和适应性免疫之间的联系

基本信息

批准号：
14207012
负责人：
ASANO Yoshihiro
金额：
$ 24.88万
依托单位：
Ehime University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14207012/
关键词：
Listeria monocytogenes Innate immunity Adaptive immunity Cytokines T cell subsets

项目摘要

Pathogenic infections lead to activation of innate immunity followed by induction of a Th1 T cell subset. However, the mechanism in this shift has not been clarified. We used DO 11.10 OVA-specific TCR-TG mouse T cell and the intracellular infectious pathogen Listeria monocytogenes (Lm) to evaluate the effect of pathogenic infections. We investigated genes that involved in the host-pathogen interaction and is required for the initiation of T cell subset differentiation in the early phase of pathogen infection. We show in this report that toll like receptor (TLR)-2, TLR-4 and MyD88 molecules are not essentially required for the induction of Th1 differentiation during Lm infection. It is also shown that genes involved in the expression of the pathogenicity of Lm are not required for the induction of Th1. We checked the mutant strain of Lm which were disrupted gene by insertion of transposon or temperature sensitive plasmid. We evaluated the mutant for determining gene(s) responsible for inducing T cell shift. We found the mutants of Lm lost either the ability of Th1 induction or the inhibition of Th2 induction, suggesting that the induction of Th1 subset and the inhibition of Th2 subset induction are separate phenomenon and are influenced by distinct genes carried by Lm genome.

病原性感染导致先天免疫的激活，随后诱导Th 1 T细胞亚群。然而，这种转变的机制尚未阐明。我们使用DO 11.10 OVA特异性TCR-TG小鼠T细胞和细胞内感染性病原体单核细胞增生李斯特菌（Lm）来评估病原性感染的效果。我们研究了参与宿主-病原体相互作用的基因，以及在病原体感染的早期阶段启动T细胞亚群分化所需的基因。我们在这份报告中表明，Toll样受体（TLR）-2，TLR-4和MyD 88分子并不是Lm感染过程中诱导Th 1分化所必需的。它还表明，参与Lm的致病性的表达的基因是不需要的诱导Th 1。我们检查了通过插入转座子或温度敏感性质粒来破坏基因的Lm突变株。我们评估了突变体用于确定负责诱导T细胞移位的基因。我们发现Lm突变体的Th 1诱导能力和Th 2诱导抑制能力均丧失，提示Lm基因组中Th 1亚群的诱导和Th 2亚群的诱导抑制是两种不同的现象，受不同基因的影响。

项目成果

期刊论文数量（58）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Shinomiya, H., et al.: "Preparation and characterization of recombinant murine p65/L-plastin expressed in E. coli and high-titer antibodies against the protein"Biosci. Biotechnol. Biochem.. (in press). (2003)

Shinomiya, H. 等人：“在大肠杆菌中表达的重组鼠 p65/L-plastin 的制备和表征以及针对该蛋白质的高滴度抗体”Biosci。

DOI：
发表时间：
期刊：
影响因子：
0
作者：
通讯作者：

Analysis of a bacterial lipopolysaccharide-activated serine kinase that posphorylates p65/L-plastin in macrophages

分析巨噬细胞中 p65/L-plastin 磷酸化的细菌脂多糖激活丝氨酸激酶

DOI：
发表时间：
期刊：
Microbiol.Immunol (印刷中)
影响因子：
0
作者：
Hagi;A.;et al.
通讯作者：
et al.

Evaluation of mitogen-induced responses in marine mammal and human lymphocytes by in-vitro exposure of butyltins and non-ortho coplanar PCBs.

DOI：
10.1016/s0269-7491(02)00155-0
发表时间：
2002-12
期刊：
Environmental pollution
影响因子：
8.9
作者：
H. Nakata;Akihito Sakakibara;M. Kanoh;S. Kudo;H. Watanabe;N. Nagai;N. Miyazaki;Y. Asano;S. Tanabe
通讯作者：
H. Nakata;Akihito Sakakibara;M. Kanoh;S. Kudo;H. Watanabe;N. Nagai;N. Miyazaki;Y. Asano;S. Tanabe

X-ray solution scattering study on the leukocyte-specific EF-hand proteins,p65/L-plastin and grancalcin.

白细胞特异性 EF-hand 蛋白、p65/L-plastin 和 grancalcin 的 X 射线溶液散射研究。