Identification of upstream gene of psoriasis and the architecture of psoriatic epidermis by crosstalk between epidermal keratinocytes and blood cells in vitro

体外表皮角质形成细胞与血细胞串扰鉴定银屑病上游基因及银屑病表皮结构

• 批准号: 24659517
• 负责人: TSUJI Hitomi
• 金额: $ 2.08万
• 依托单位: Asahikawa Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659517/
• 关键词: 尋常性乾癬; 共培養; 3次元培養表皮角化細胞; クロストーク; SLURP-2; 3次元培養; 上流遺伝子

Psoriasis vulgaris is one of chronic inflammatory skin diseases. Its pathogenesis remains to be completely elucidated. SLURP-2, a secreted Ly-6/uPAR related protein-2 is a secretory protein, which is significantly upregulated in the psoriatic lesion. SLURP-2/FLAG expression vector was constructed, followed by the transfection into normal human epidermal keratinocytes. The transfected keratinocytes showed the overexpression of the proinflammatory cytokines or antimicrobial peptides that are upregulated in psoriasis. Those cells were co-cultured with blood cells from healthy subjects. The epidermal keratinoyctes in three-dimensional culture system, which were cultivated with the supernatant of co-culture, resulted in acanthotic epidermal architecture. These findings suggest that SLURP-2 may be involved in the pathophysiology of psoriasis.

寻常型银屑病是一种慢性炎症性皮肤病。其发病机制尚未完全阐明。SLURP-2是一种分泌型Ly-6/uPAR相关蛋白-2，在银屑病皮损中表达显著上调。构建SLURP-2/FLAG表达载体，转染正常人表皮角质形成细胞。转染的角质形成细胞表现出在银屑病中上调的促炎细胞因子或抗菌肽的过表达。这些细胞与来自健康受试者的血细胞共培养。三维培养体系中的表皮角质形成细胞与共培养上清液共同培养，形成棘层表皮结构。这些发现表明，SLURP-2可能参与银屑病的病理生理。