Development of activator for skin function using bone marrow mesenchymal stem cell mobilizer

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Using skin transplantation system on GFP-transgenic bone marrow-transplanted mouse, bone marrow-derived mesenchymal stem cells (MSCs) in the skin graft were evaluated by flow cytometry analysis. As a result, bone marrow -derived MSCs in the skin graft increased time dependently, and reached to ~1 % of all cell population in the skin graft at day 7 after the engraftment. Further precise investigation revealed that particular cell population (PDGFRα+/SSEA3+) in MSCs is a pluripotent stem cells harboringkeratinocyte-differentiation potential. Furthermore, intravenous administration of HMGB1, which had been shown to mobilize bone marrow MSCs into the circulation, showed potent activities to improve function of the skin graft by increasing MSC numbers, enhancing angiogenesis, and suppressing inflammation in the skin graft.