Basic research for inducing epithelial regeneration by bone marrow-derived epithelial cells

骨髓源性上皮细胞诱导上皮再生的基础研究

• 批准号: 19390295
• 负责人: TAMAI Katsuto
• 金额: $ 11.98万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19390295/
• 关键词: 骨髄間葉系幹細胞; 先天性表皮水疱症; 骨髄移植; 骨髄由来表皮細胞; 表皮幹細胞; 先天性表表皮水疱; Green fluorescent protein(GFP); VII型コラーゲン; 表皮水疱症; PDGFRα; CD44; Green fluorescent protein (GFP)

We demonstrated that detached RDEB epithelia recruit platelet-derived growth factor receptor alpha (PDGFRα)-positive bone marrow cells (PDGFRα+ BMCs) via circulation, and that the PDGFRα+ BMCs contain particular population to provide epithelial cells expressing keratin 5 in the RDEB skin by differentiation of those bone marrow-derived PDGFRα-positive cells. Furthermore, the PDGFRα-positive BMCs were shown to be undifferentiated non-hematopoietic cell populations, and to reside particularly on and around surface of the bone and trabeculae in bone marrow by intravital two-photon imaging.

我们证明，分离的RDEB上皮细胞通过循环招募血小板源性生长因子受体α（PDGFRα）阳性骨髓细胞（PDGFRα+ BMCs），PDGFRα+ BMCs含有特定的群体，通过这些骨髓源性PDGFRα阳性细胞的分化，在RDEB皮肤中提供表达角蛋白5的上皮细胞。此外，PDGFRα阳性BMCs是未分化的非造血细胞群，并且通过活体双光子成像显示其特别位于骨表面和周围以及骨髓中的小梁。

项目成果

Basal keratinocyte-targeting gene delivery system for in vivo EB gene therapy

用于体内 EB 基因治疗的基础角质形成细胞靶向基因递送系统

• 发表时间: 2007
• 作者: Shimbo T;Kawachi M;Saga K;Fujita H;Yamazaki T;Tamai K;Kaneda Y;Katsuto Tamai
• 通讯作者: Katsuto Tamai

Controlled Release of Bone Morphogenetic Protein-2 Enhances Recruitment of Osteogenic Progenitor Cells for De Novo Generation of Bone Tissue

• DOI: 10.1089/ten.tea.2009.0322
• 发表时间: 2010-04-01
• 期刊: TISSUE ENGINEERING PART A
• 影响因子: 4.1
• 作者: Kimura, Yu;Miyazaki, Nobuhiko;Tabata, Yasuhiko
• 通讯作者: Tabata, Yasuhiko

Bone marrow stem cell therapy for epidermolysis bullosa

骨髓干细胞治疗大疱性表皮松解症

• 发表时间: 2007
• 作者: Shimbo T;Kawachi M;Saga K;Fujita H;Yamazaki T;Tamai K;Kaneda Y;Katsuto Tamai;Katsuto Tamai
• 通讯作者: Katsuto Tamai

Bone marrow is an essential source of keratinocytes in the grafted skin

骨髓是移植皮肤角质形成细胞的重要来源

• 发表时间: 2007
• 作者: Shimbo T;Kawachi M;Saga K;Fujita H;Yamazaki T;Tamai K;Kaneda Y;Katsuto Tamai;Katsuto Tamai;Katsuto Tamai;Katsuto Tamai
• 通讯作者: Katsuto Tamai

骨髄由来幹細胞による皮膚再生メカニズム

骨髓干细胞的皮肤再生机制

• 发表时间: 2009
• 作者: Kimura Y;Miyazaki N;Hayashi N;Otsuru S;Tamai K;Kaneda Y;Tabata Y.;玉井克人
• 通讯作者: 玉井克人