Development of in vitro culture for peripheral B cell differentiation into plasma cell

外周B细胞分化为浆细胞的体外培养方法的开发

• 批准号: 24659587
• 负责人: KOBAYASHI Takaaki
• 金额: $ 2.33万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659587/
• 关键词: 移植・再生医療; Ｂリンパ球; 形質細胞; 培養; HLA抗体; 抗体

We attempted to develop a novel in vitro culture assay for proliferation and differentiation of peripheral B cells into plasma cells. Using 3 step culture method, the difference in action points (in the process of B cell differentiation) between clinical available immunosuppressive agents such as calcineurin inhibitor (CNI), mTOR-inhibitor (mTOR-I), anti-metabolite and steroid was clarified. The results showed anti-metabolite, mTOR-I and steroid could suppress B cells in the early, middle and late phase, respectively. Effective method for HLA antibody secretion in the B cell culture system has been established using APRIL (A proliferation-inducing ligand), BAFF (B cell activating factor), mitogen-stimulated T cell secretion and adipose-derived mesenchymal stem cells. We can move on to the next step to evaluate early diagnosis of chronic antibody mediated rejection.

我们试图开发一种新的体外培养实验，用于外周B细胞向浆细胞的增殖和分化。采用三步培养法，明确了临床可用的钙调磷酸酶抑制剂（CNI）、mtor抑制剂（mTOR-I）、抗代谢物和类固醇等免疫抑制剂在B细胞分化过程中作用点的差异。结果显示，抗代谢物、mtor - 1和类固醇分别在早期、中期和晚期抑制B细胞。利用A增殖诱导配体（APRIL）、B细胞活化因子（BAFF）、丝裂原刺激T细胞分泌和脂肪源间充质干细胞，建立了B细胞培养系统中HLA抗体分泌的有效方法。我们可以继续下一步来评估慢性抗体介导的排斥反应的早期诊断。

项目成果

Comparative analysis of drug action on B-cell proliferation and differentiation for mycophenolic acid, everolimus, and prednisolone.

• DOI: 10.1097/01.tp.0000441826.70687.f6
• 发表时间: 2014-02-27
• 期刊: Transplantation
• 影响因子: 6.2
• 作者: Haneda M;Owaki M;Kuzuya T;Iwasaki K;Miwa Y;Kobayashi T
• 通讯作者: Kobayashi T

リコンビナント抗血液型A型糖鎖糖鎖IgG抗体の作成

重组抗A型血糖链糖链IgG抗体的创建

• 发表时间: 2013
• 作者: 羽根田正隆;三輪祐子;岩崎研太;小林孝彰
• 通讯作者: 小林孝彰

In Vitro Differentiation of Naive/Memory B Cells into Plasma Cells : Difference in Mode of Drug Action between Mycophenolic Acid and Everolimus

幼稚/记忆 B 细胞体外分化为浆细胞：麦考酚酸和依维莫司药物作用模式的差异

• 发表时间: 2013
• 作者: 羽根田正隆;成田由紀子;三輪祐子;岩崎研太;小林孝彰;Yoshiko Matsuda,Yuko Miwa,Kenta Iwasaki,Masataka Haneda,Takaaki Kobayashi;Yoshiko Matsuda,Yuko Miwa,Kenta Iwasaki,Masataka Haneda,Takaaki Kobayashi;M. Haneda,M. Owaki,K. Iwasaki,Y. Miwa,Y. Watarai,K. Uchida,T. Kobayashi
• 通讯作者: M. Haneda,M. Owaki,K. Iwasaki,Y. Miwa,Y. Watarai,K. Uchida,T. Kobayashi

In vitro B cell differentiation assayを用いたDSA検出法の検討

体外 B 细胞分化试验 DSA 检测方法的检验

• 发表时间: 2012
• 作者: 羽根田正隆;黒木聖久;成田由紀子;大脇麻未;葛谷孝文;岩崎研太;渡井至彦;打田和治;小林孝彰
• 通讯作者: 小林孝彰

移植前脱感作療法は長期のde novo HLA抗体産生を抑制するか?

移植前脱敏治疗是否会抑制长期 HLA 抗体从头产生？

• 发表时间: 2012
• 作者: 黒木聖久;小林孝彰 他
• 通讯作者: 小林孝彰 他