Development of in vitro culture for peripheral B cell differentiation into plasma cell

点击翻译按钮获取中文摘要

We attempted to develop a novel in vitro culture assay for proliferation and differentiation of peripheral B cells into plasma cells. Using 3 step culture method, the difference in action points (in the process of B cell differentiation) between clinical available immunosuppressive agents such as calcineurin inhibitor (CNI), mTOR-inhibitor (mTOR-I), anti-metabolite and steroid was clarified. The results showed anti-metabolite, mTOR-I and steroid could suppress B cells in the early, middle and late phase, respectively. Effective method for HLA antibody secretion in the B cell culture system has been established using APRIL (A proliferation-inducing ligand), BAFF (B cell activating factor), mitogen-stimulated T cell secretion and adipose-derived mesenchymal stem cells. We can move on to the next step to evaluate early diagnosis of chronic antibody mediated rejection.