Comprehensive metabolome analysis of pancreatic cancer for identifying the pancreatic cancer-specific metabolic traits

胰腺癌的综合代谢组分析，用于识别胰腺癌特异性代谢特征

• 批准号: 24659612
• 负责人: YACHIDA Shinichi
• 金额: $ 2.41万
• 依托单位: National Center of Neurology and Psychiatry
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659612/
• 关键词: 膵臓癌; メタボローム; メタボローム解析; 膵癌

The pancreatic cancer is a devastating disease. We have performed the comprehensive metabolome analysis method based on capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS), which allows quantitative analysis of thousands of charged metabolites. This approach was applied to simultaneous analysis of metabolite levels in tumor and normal counterparts obtained from 24 patients with pancreatic cancer. Enhanced glycolysis was found in tumor tissues, indicating the Warburg effect, which is the enhanced conversion of glucose to lactate observed in tumor cells, even in the presence of normal levels of oxygen. In addition, quantification of hundred metabolites resulted in the identification of pancreatic cancer-specific metabolic traits. The amino acid concentrations were increased in tumor tissues. These data will apply into advanced imaging methods and blood tests to detect pancreatic cancer-specific metabolomes, which offer hope for such non-invasive early detection

胰腺癌是一种毁灭性的疾病。 我们已经进行了全面的代谢物组分析方法的基础上，毛细管电泳飞行时间质谱（CE-TOFMS），它允许定量分析数以千计的带电代谢物。 这种方法被应用于同时分析的代谢物水平在肿瘤和正常的同行从24例胰腺癌患者。 在肿瘤组织中发现糖酵解增强，表明瓦尔堡效应，即即使在正常水平的氧气存在下，在肿瘤细胞中观察到葡萄糖向乳酸盐的转化增强。 此外，对上百种代谢物的定量导致了胰腺癌特异性代谢特征的鉴定。 肿瘤组织中氨基酸浓度升高。 这些数据将应用于先进的成像方法和血液测试，以检测胰腺癌特异性代谢组，这为这种非侵入性早期检测提供了希望

项目成果

Small cell and large cell neuroendocrine carcinomas of the pancreas are genetically similar and distinct from well-differentiated pancreatic neuroendocrine tumors.

• DOI: 10.1097/pas.0b013e3182417d36
• 发表时间: 2012-02
• 期刊: The American journal of surgical pathology
• 作者: Yachida S;Vakiani E;White CM;Zhong Y;Saunders T;Morgan R;de Wilde RF;Maitra A;Hicks J;Demarzo AM;Shi C;Sharma R;Laheru D;Edil BH;Wolfgang CL;Schulick RD;Hruban RH;Tang LH;Klimstra DS;Iacobuzio-Donahue CA
• 通讯作者: Iacobuzio-Donahue CA

Clinicopathology of recurrent hepatocellular carcinomas after radiofrequency ablation treated with salvage surgery

• DOI: 10.1111/hepr.12223
• 发表时间: 2014-10-01
• 期刊: HEPATOLOGY RESEARCH
• 影响因子: 4.2
• 作者: Yamamoto, Naoki;Okano, Keiichi;Suzuki, Yasuyuki
• 通讯作者: Suzuki, Yasuyuki

Immunohistochemically Detected Expression of 3 Major Genes (CDKN2A/p16, TP53, and SMAD4/DPC4) Strongly Predicts Survival in Patients With Resectable Pancreatic Cancer

• DOI: 10.1097/sla.0b013e3182827a65
• 发表时间: 2013-08-01
• 期刊: ANNALS OF SURGERY
• 影响因子: 9
• 作者: Oshima, Minoru;Okano, Keiichi;Yachida, Shinichi
• 通讯作者: Yachida, Shinichi

Novel therapeutic approaches in pancreatic cancer based on genomic alterations

基于基因组改变的胰腺癌新治疗方法

• 发表时间: 2012
• 期刊: Curr Pharm Des
• 作者: Yachida S;White CM;Naito Y;Zhong Y;Brosnan JA;Macgregor-Das AM;Morgan RA;Saunders T;Laheru DA;Herman JM;Hruban RH;Klein AP;Jones S;V elculescu V;Wolfganag CL;Iacobuzio-Donahue CA;Yachida S
• 通讯作者: Yachida S

Heterogeneity of Pancreatic Cancer Metastases in a Single Patient Revealed by Quantitative Proteomics

• DOI: 10.1074/mcp.m114.038547
• 发表时间: 2014-11-01
• 期刊: MOLECULAR & CELLULAR PROTEOMICS
• 影响因子: 7
• 作者: Kim, Min-Sik;Zhong, Yi;Pandey, Akhilesh
• 通讯作者: Pandey, Akhilesh