Development of novel methods for imaging of bone marrow utilizing fluorescence techniques and spatiotemporal analysis of bone metastasis

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To elucidate the molecular mechanisms of interaction between cancer cells and microenvironments which determine the osteolytic/ osteogenic tendencies of bone metastases of cancer, series of experiments were performed to establish an in vivo imaging system by which 4 kind of cells, cancer cells, osteoclasts, osteoblasts and osteocytes in bone marrow can be analyzed simultaneously at multiple time points. Cancer cells expressing fluorescent proteins were implanted to mice and bone metastasis system of osteolytic prostate cancer was established. In vivo fluorescence imaging for osteoclast was also performed.