In vivo analysis of the interaction between RANK and RANKL by using innovative fluorescent probes

利用荧光蛋白互补部分结合的荧光检测系统(BIFC)，构建了检测RANK和RANKL相互作用所需的基因，RANK和RANKL是破骨细胞形成所必需的蛋白，而破骨细胞在骨吸收中起核心作用。在将这两个基因导入细胞的初步实验中，在RANK和RANKL可以结合的条件下检测到荧光。

By utilizing a fluorescence detection system by binding of complementary parts of a fluorescent protein(BiFC), the genes needed for the detection of the interaction of RANK and RANKL, proteins necessary for the formation of osteoclasts whoch have a central role in bone resorption, were constructed. In a preliminary experiment in which these two genes were introduced into cells, fluorescence was detected under the condition when RANK and RANKL could bind together.