Investigation for establishment for a novel intervention to protect kidney against hypoxic injury by inducing metabolic reprogramming by manipulating the transcription factor HIF-1 activity

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In order to established a novel method to protect kidney from ischemic injury, we explored an intervention by manipulate the transcription factor HIF-1 activity with the small molecular compound n-propyl gallate (nPG). We demonstrated that forced activation of hypoxia-inducible factor 1(HIF-1) by the exogenous small molecule n-propyl gallate (nPG) induced metabolic reprograming from cellular energy generation dependent on OXPHOS in mitochondria to glycolysis-dominant one in the primary cultured tubular and glomerular cells. Moreover, we indicated that nPG pretreatment induced HIF-1 activation and expression of glycolytic enzymes and conferred tolerance against hypoxia-induced cell death of cells derived from kidney.