Analysis of N-glycans associated with diabetic retinopathy

聚糖是承载生物信息的生物高分子，是继核酸和蛋白质之后的第三大类细胞大分子。糖基化是一个重要的翻译后修饰过程，改变了蛋白质的功能。在这项研究中，我们分析了非增殖性玻璃体视网膜疾病（如视网膜前膜和黄斑孔）患者玻璃体液中n-聚糖的分布（n=20）。玻璃体样品中n -聚糖的浓度明显低于血浆样品。在这两个样品中，n -聚糖都是常见的，结构为[(Hex)2 (HexNAc)2 (NeuAc)2 + (Man)3(GlcNAc)2]。

Glycans are biopolymers bearing biological information and regarded as the third major class of cellular macromolecules, following nucleic acids and proteins. Glycosylation is an important process of post-translational modification that changes the functions of proteins. In this study, we analyzed the profile of N-glycans in human vitreous fluid obtained from patients with non-proliferative vitreoretinal diseases such as epiretinal membrane and macular hole (n=20). The concentration of N-glycans in vitreous samples was significantly lower compared with those in plasma samples. In both samples, predominant N-glycan was common and the structure is [(Hex)2 (HexNAc)2 (NeuAc)2 + (Man)3(GlcNAc)2].