Analysis of N-glycans associated with diabetic retinopathy

与糖尿病视网膜病变相关的 N-聚糖分析

• 批准号: 24659754
• 负责人: ISHIDA Susumu
• 金额: $ 2.41万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659754/
• 关键词: 糖尿病網膜症; 糖鎖; グライコブロッティング

Glycans are biopolymers bearing biological information and regarded as the third major class of cellular macromolecules, following nucleic acids and proteins. Glycosylation is an important process of post-translational modification that changes the functions of proteins. In this study, we analyzed the profile of N-glycans in human vitreous fluid obtained from patients with non-proliferative vitreoretinal diseases such as epiretinal membrane and macular hole (n=20). The concentration of N-glycans in vitreous samples was significantly lower compared with those in plasma samples. In both samples, predominant N-glycan was common and the structure is [(Hex)2 (HexNAc)2 (NeuAc)2 + (Man)3(GlcNAc)2].

聚糖是一种具有生物信息的生物聚合物，被认为是继核酸和蛋白质之后的第三大类细胞大分子。 糖基化是蛋白质翻译后修饰的一个重要过程，它改变了蛋白质的功能。 在这项研究中，我们分析了从患有非增殖性玻璃体视网膜疾病（如视网膜前膜和黄斑裂孔）的患者（n=20）中获得的人玻璃体液中N-聚糖的分布。 玻璃体样本中N-聚糖的浓度显著低于血浆样本中的浓度。 在两种样品中，主要N-聚糖是常见的，结构为[（Hex）2（HexNAc）2（NeuAc）2 +（Man）3（GlcNAc）2]。

项目成果

A comparison of N-glycan profiles in human plasma and vitreous fluid

• DOI: 10.1007/s00417-014-2671-x
• 发表时间: 2014-06
• 期刊: Graefe's Archive for Clinical and Experimental Ophthalmology
• 作者: Saori Inafuku;K. Noda;M. Amano;Tetsu Ohashi;Chikako Yoshizawa;W. Saito;Atsuhiro Kanda;S. Nishimura;S. Ishida

Profile analysis of glycans in human vitreous

人玻璃体中聚糖的概况分析

• 发表时间: 2013
• 作者: Takashina S;Noda K;Amano M;Saito W;Nishimura S;Ishida S
• 通讯作者: Ishida S