New analysis of inner ear pharmacology

内耳药理学的新分析

• 批准号: 24659752
• 负责人: OGAWA Kaoru
• 金额: $ 2.33万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659752/
• 关键词: 内耳; 薬物動態; 鼓室内投与; 徐放作用; ドラッグデリバリー

Methods 1)Luciferin delivered into the inner ears of GFAP-Luc transgenic mice reacted with luciferase in GFAP-expressing cells in the cochlear spiral ganglion, resulting in photon bioluminescence.Both luciferin and hyaluronic acid (HA) were injected through the left side of ear. We changed the concentration and viscosity of HA. Signals were obtained in the left side of ear in GFAP-luc mice through the IVIS system. 2) Strong intensity noise exposed into male Guinea pigs for two hours. Combination of dexamethasone and HA was administrated into the ear.Results 1) Signal does not depend on the viscosity of hyaluronic acid of inner ear. One reason is that high viscosity of HA can not go through the round window membrane. We considered that the technical problem was involved.2)The question is "Is the combination of steroid and HA successful or not?"ABR was tested before and after treatment.

方法1)将荧光素注入GFAP-Luc转基因小鼠内耳，在耳蜗螺旋神经节表达gfap的细胞中与荧光素酶发生反应，产生光子生物发光。通过左耳注射荧光素和透明质酸（HA）。我们改变了HA的浓度和粘度。通过IVIS系统在gmap -luc小鼠耳左侧获得信号。2)强强度噪声暴露于雄性豚鼠体内2小时。地塞米松联合血凝素经耳给药。结果1)信号不依赖于内耳透明质酸黏度。原因之一是高粘度的透明质酸无法通过圆窗膜。我们认为涉及到技术问题。2)问题是“类固醇和血凝素的结合是否成功？”治疗前后分别检测ABR。

项目成果

（総説）耳の病気に対する耳寄りな治療法を紹介！感音難聴に対する薬物・遺伝子の内耳局所投与の実現のために

(评论) 介绍针对耳部疾病的对耳朵友好的治疗方法！

• 发表时间: 2013
• 期刊: 日本薬理学会誌
• 作者: 神崎晶;神崎晶
• 通讯作者: 神崎晶

Novel in vivo imaging analysis of an inner ear (spiral ganglion) drug delivery system in mice.

小鼠内耳（螺旋神经节）药物输送系统的新型体内成像分析。

• 作者: Kanzaki S;Fujioka M;Yasuda A;Shibata S;Nakamura M;Okano HJ;Okano H;Ogawa K
• 通讯作者: Ogawa K

Novel in vivo imaging analysis of an inner ear (spiral ganglion) drug delivery system in mice

小鼠内耳（螺旋神经节）药物输送系统的新型体内成像分析

• 发表时间: 2013
• 作者: Kanzaki S;Fujioka M;Yasuda A;Shibata S;Nakamura M;Okano HJ;Okano H;Ogawa K
• 通讯作者: Ogawa K

Feasibility of drug and gene therapy for sensorineural hearing loss.

药物和基因治疗感音神经性听力损失的可行性。

• 发表时间: 2013
• 期刊: Nihon Yakurigaku Zasshi
• 作者: 神崎晶;神崎晶;Kanzaki S
• 通讯作者: Kanzaki S

Drug and Gene Delivery into Inner Ear for Neural and Neuroepithelial cells

将药物和基因递送至内耳的神经和神经上皮细胞

• 发表时间: 2013
• 作者: Kanzaki S;Fujioka M;Yasuda A;Shibata S;Nakamura M;Okano HJ;Okano H;Ogawa K;Kanzaki S
• 通讯作者: Kanzaki S