New analysis of inner ear pharmacology

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Methods 1)Luciferin delivered into the inner ears of GFAP-Luc transgenic mice reacted with luciferase in GFAP-expressing cells in the cochlear spiral ganglion, resulting in photon bioluminescence.Both luciferin and hyaluronic acid (HA) were injected through the left side of ear. We changed the concentration and viscosity of HA. Signals were obtained in the left side of ear in GFAP-luc mice through the IVIS system. 2) Strong intensity noise exposed into male Guinea pigs for two hours. Combination of dexamethasone and HA was administrated into the ear.Results 1) Signal does not depend on the viscosity of hyaluronic acid of inner ear. One reason is that high viscosity of HA can not go through the round window membrane. We considered that the technical problem was involved.2)The question is "Is the combination of steroid and HA successful or not?"ABR was tested before and after treatment.