Establishment of salivary gland regeneration method using salivary gland tissue-originated spheroid (STOS)

我们研究了分离的E13.5胚胎颌下腺(SMG)细胞在I型胶原凝胶中的体外再生能力。由此产生的结构类似于天然唾液腺。第7天开始出现大的囊样结构，可能是由于导管肿胀所致，第14天时它们变大并增多。在再生的唾液腺中加入纤维连接蛋白和抗整合素抗体，明显抑制了囊的形成和扩大，AQP5的表达和定位集中在管腔一侧，细胞极性与自然唾液腺相似。相反，抗整合素抗体诱导了囊泡的形成和增大。综上所述，这些数据表明，分离的SMGs可以再生唾液腺结构，整合素和细胞外基质之间的特异性相互作用对于再生唾液腺的分化和发育是必不可少的。

We demonstrated the regenerative capability of dissociated E13.5 embryonic submandibular gland (SMG) cells in vitro in collagen type I gels. The resulting structures were similar to native salivary glands. Large cyst-like structures, probably due to swollen ducts, began to appear on day 7, and they enlarged and increased in number by day 14. Through the addition of fibronectin and an anti- integrin antibody into the regenerate salivary glands, cyst formation and enlargement were clearly inhibited, and the expression and localization of AQP5 was concentrated on the lumen side with cell polarity similar to native salivary glands. In contrast, anti- integrin antibodies induced cyst formation and enlargement. Together, these data indicate that dissociated SMGs can regenerate salivary gland structure, and specific interactions between integrins and extracellular matrices are essential for regenerate salivary gland differentiation and development.