A pilot study for visualizing diurnal hormonal secretion pattern within a hair

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The hormones shows a characteristic diurnal secretion pattern, the higher in the morning and lower in the evening. This diurnal hormonal secretion in the blood might be recorded within a hair just like as "an annual ring of tree," because the hair growth, which is about 0.3 mm every day, is involved one of the most active cellular activity in the body. The challenge of this study is to visualize this diurnal pattern by using biochemical analysis, such as protein staining and immunostaining. This objective has yet to be achieved so far. However, there accumulated the techniques and knowledge to reach a final goal, such as development of making hair section technique and protein staining procedure for the hair section, and observation of detailed hormonal diurnal patter, especially during night through morning.