OPR Protein-mediated Control and Adaption of Chloroplast Gene Expression in Chlamydomonas

OPR 蛋白介导的衣藻叶绿体基因表达的控制和适应

• 批准号: 396655363
• 负责人: Privatdozentin Dr. Alexandra-Viola Bohne
• 金额: --
• 依托单位: Bereich Pflanzenwissenschaften
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/396655363?language=en
• 关键词: OPR; Protein; mediated; Control; Adaption

Due to the endosymbiotic origin of chloroplasts, many of the proteins constituting the multisubunit complexes of the thylakoid membrane are nowadays encoded in the nucleus while others are encoded in the chloroplast itself. Chloroplast gene expression therefore needs to be coordinated with the nuclear one to allow a balanced assembly of protein complexes. In addition, gene expression of the organelle requires an adaption to a changing environment, in particular light, to meet the demand of the cell. To date, the predominant role of Octotricopeptide Repeat proteins (OPRs) as key players for the posttranscriptional control of chloroplast gene expression in green algae is just emerging. The RNA targets of the majority of OPRs encoded by the algal genome as well as their molecular working modes are unknown. Moreover, the specific interaction of OPRs with their plastid target RNAs makes them ideal candidates as regulators of chloroplast gene expression for an adaption to changing light conditions. As photosystem II (PSII) is most susceptible to photodamage, the focus of the proposed project lies on OPRs with confirmed or expected functions in the expression of PSII subunits in the model organism Chlamydomonas reinhardtii. To gain further important insights into the OPR-mediated control and regulation of chloroplast gene expression, we aim at (I) elucidating the precise molecular working mode of two OPRs involved in the stabilization of two mRNAs encoding PSII subunits, (II) identify new OPRs involved in PSII biogenesis and (III) investigate the impact of these OPRs on light-dependent adaptive processes of the organelle.

由于叶绿体的内共生起源，构成类囊体膜多亚基复合物的许多蛋白质现在在细胞核中编码，而其他蛋白质则在叶绿体本身中编码。因此，叶绿体基因的表达需要与核基因的表达相协调，以平衡蛋白质复合物的组装。此外，细胞器的基因表达需要适应不断变化的环境，特别是光，以满足细胞的需求。迄今为止，在绿色藻类中，三肽重复蛋白（OPRs）作为叶绿体基因表达的转录后调控的关键参与者的主导作用才刚刚出现。藻类基因组编码的大多数OPR的RNA靶标以及它们的分子工作模式是未知的。此外，OPR与其质体靶RNA的特异性相互作用使其成为叶绿体基因表达调节剂的理想候选者，以适应不断变化的光条件。由于光系统II（PSII）是最容易受到光损伤，拟议的项目的重点在于OPR与确认或预期的功能，在模式生物莱茵衣藻PSII亚基的表达。为了进一步深入了解OPR介导的叶绿体基因表达的控制和调节，我们的目标是：（I）阐明两个OPR参与编码PSII亚基的两个mRNA的稳定化的精确分子工作模式，（II）确定新的OPR参与PSII生物合成和（III）调查这些OPR对光依赖的细胞器适应过程的影响。