Development of a nanopore trapping method for identification of single-viruses

这项研究旨在开发一种纳米孔捕集方法，用于通过横向电流测量来识别分析物。结果表明，通过控制电泳电压，单个粒子可以在纳米孔中被重复捕获/解禁。在捕获时，由于被捕获的粒子部分阻止了离子在孔隙中的传输，通过孔隙的离子电流下降，其电信号被发现有助于区分表面电荷状态。横向电流测量也是通过在纳米孔中嵌入两个电极来实现的。通过测量通过这些电极的电流，电流显示出与气孔处的粒子陷阱同步的脉冲式变化。这是由于电极表面双电层的性质发生了变化。总而言之，横向电流法可以是一种新的方法，它不是根据分析物的体积，而是通过其他性质，如表面电荷密度来识别分析物。

This research aims to develop a nanopore trapping method for identification of analytes via transverse current measurements. It is demonstrated that single-particles can be trapped/detrapped repeatedly at a nanopore by electrophoretic voltage control. Upon trapping, ion current through a pore drops due to partial blockade of ion transport through the pore by the trapped particles, the electrical signals of which are found useful in discriminating the surface charge status. Transverse current measurements are also performed by using two electrodes embedded in a nanopore. Measuring the current through these electrodes, the current shows pulse-like changes synchronously to the particle trap at a pore. This is attributable to a change in the properties of electric double layers on electrodes' suface. Overall, it is shown that the transverse current approach can be a new method capable of identifying analytes not by their volume but other properties such as surface charge densities.