Development of a nanopore trapping method for identification of single-viruses

开发用于识别单一病毒的纳米孔捕获方法

• 批准号: 25600061
• 负责人: TSUTSUI Makusu
• 金额: $ 2.58万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2013
• 资助国家: 日本
• 起止时间: 2013-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-25600061/
• 关键词: ナノポア; センサー; 電気泳動; ナノ粒子

This research aims to develop a nanopore trapping method for identification of analytes via transverse current measurements. It is demonstrated that single-particles can be trapped/detrapped repeatedly at a nanopore by electrophoretic voltage control. Upon trapping, ion current through a pore drops due to partial blockade of ion transport through the pore by the trapped particles, the electrical signals of which are found useful in discriminating the surface charge status. Transverse current measurements are also performed by using two electrodes embedded in a nanopore. Measuring the current through these electrodes, the current shows pulse-like changes synchronously to the particle trap at a pore. This is attributable to a change in the properties of electric double layers on electrodes' suface. Overall, it is shown that the transverse current approach can be a new method capable of identifying analytes not by their volume but other properties such as surface charge densities.

本研究的目的是发展一种纳米孔捕获方法，通过横向电流测量来识别分析物。 它表明，单粒子可以被捕获/脱陷重复在纳米孔通过电泳电压控制。 在捕获时，通过孔的离子电流由于被捕获的颗粒通过孔的离子传输的部分阻断而下降，发现其电信号可用于区分表面电荷状态。 还通过使用嵌入纳米孔中的两个电极来执行横向电流测量。 测量通过这些电极的电流，电流显示出与孔隙处的颗粒陷阱同步的脉冲状变化。 这是由于电极表面双电层的性质发生了变化。 总体而言，它表明，横向电流的方法可以是一种新的方法，能够识别分析物的体积，但其他属性，如表面电荷密度。

项目成果

Controlling particle position using a nanopore trapping method

使用纳米孔捕获方法控制颗粒位置

• 发表时间: 2013
• 作者: Y. Maeda;M. Tsutsui;K. Doi;S. Kawano;T. Kawai;M. Taniguchi
• 通讯作者: M. Taniguchi

Trapping and identifying single-nanoparticles using a low-aspect-ratio nanopore

• DOI: 10.1063/1.4813084
• 发表时间: 2013-07-01
• 期刊: APPLIED PHYSICS LETTERS
• 影响因子: 4
• 作者: Tsutsui, Makusu;Maeda, Yoichi;Taniguchi, Masateru
• 通讯作者: Taniguchi, Masateru

ナノポアトラップ法による単一粒子の識別

纳米孔陷阱法单颗粒识别

• 发表时间: 2013
• 作者: 前田陽一;筒井真楠;土井謙太郎;川野聡恭;川合知二;谷口正輝
• 通讯作者: 谷口正輝