Membrane translocation and chaperone-dependent folding of the lipase A ofPseudomonas aeruginosa (A10#)

铜绿假单胞菌 (A10) 脂肪酶 A 的膜易位和分子伴侣依赖性折叠

• 批准号: 397451917
• 金额: --
• 依托单位: Lehrstuhl für Biochemie I
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/397451917?language=en
• 关键词: Membrane; translocation; chaperone; dependent; folding

Extracellular lipases are recognized as virulence factors of Pseudomonas aeruginosa, however their chap-erone-mediated folding at the lipid membrane interface is not understood. Here, we set out to elucidate how the membrane environment steers lipase biogenesis. We will employ the newly reconstituted system and in vivo experiments to study (i) how the membrane-anchored LipH chaperone contributes to the lipase A translocation, (ii) whether lipids or membrane-associated proteins regulate the lipase:LipH assembly, and (iii) how the lipase folding is influenced by the crowded and dynamic environment at the membrane interface.

胞外脂肪酶被认为是铜绿假单胞菌的毒力因子，但其在脂膜界面的折叠机制尚不清楚。在这里，我们着手阐明膜环境如何引导脂肪酶的生物发生。我们将采用新构建的系统和体内实验来研究(i)膜锚定的LipH伴侣如何促进脂肪酶A易位，（ii）脂质或膜相关蛋白是否调节脂肪酶：LipH组装，以及（iii）脂肪酶折叠如何受到膜界面拥挤和动态环境的影响。