Development of a Subtraction Sequencing method for obtaining genomic information of parsites

开发用于获取寄生虫基因组信息的减法测序方法

• 批准号: 25670200
• 负责人: MATSUZAKI Motomichi
• 金额: $ 2.5万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2013
• 资助国家: 日本
• 起止时间: 2013-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-25670200/
• 关键词: ゲノム; 超並列シーケンシング

The objective of the present study was to develop a subtraction sequencing method for obtaining genomic information of unicellular parasites. Escherichia coli and Saccharomyces cerevisiae DNAs were used as models for a host and parasite genome, respectively. The latter DNA was concentrated by subtracting the former DNA from the DNA mixture with biotinated E. coli library and streptavidin magnetic beads. The concentration was verified with quantitative PCR. Now this method is proven as feasible, but further elaboration will be required for practical use.

本研究的目的是建立一种获得单细胞寄生虫基因组信息的差减测序方法。大肠杆菌和酿酒酵母DNA分别用作宿主和寄生虫基因组的模型。用生物素化的E. coli文库和链霉亲和素磁珠。用定量PCR验证浓度。现在这种方法被证明是可行的，但在实际应用中还需要进一步的阐述。