Identification of noncoding RNAs that regulate macrophage dynamics in chronic inflammation

鉴定调节慢性炎症中巨噬细胞动态的非编码 RNA

• 批准号: 25670381
• 负责人: MANABE Ichiro
• 金额: $ 2.41万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2013
• 资助国家: 日本
• 起止时间: 2013-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-25670381/
• 关键词: 非翻訳RNA; マクロファージ

Macrophages are the key effector cells in chronic inflammatory processes in cardiovascular disease. They alter their phenotypes and functions during the processes. This study aimed to identify the noncoding RNAs that control dynamic changes in macrophage functions. We identified more than 1,000 novel long noncoding RNAs (lncRNAs0 expressed in macrophages. We also found that some of them bind to the PU.1 transcriptional regulatory complexes. Among them two lncRNAs are induced by LPS in cultured macrophages. They are also induced by tissue injury in macrophages in in vivo settings. Knocking down the lncRNAs altered the response of macrophages to LPS, suggesting they control macrophage functions.

巨噬细胞是心血管疾病慢性炎症过程中的关键效应细胞。它们在此过程中改变其表型和功能。本研究旨在鉴定控制巨噬细胞功能动态变化的非编码RNA。我们发现了超过1，000种在巨噬细胞中表达的新型长链非编码RNA（lncRNA 0）。我们还发现，其中一些结合到PU.1转录调控复合物。其中两种lncRNA是由LPS在培养的巨噬细胞中诱导的。它们也由体内巨噬细胞中的组织损伤诱导。敲低lncRNA改变了巨噬细胞对LPS的反应，表明它们控制着巨噬细胞的功能。

项目成果

炎症性マクロファージにおける長鎖ノンコーディングRNAの役割

长链非编码RNA在炎症巨噬细胞中的作用

• 发表时间: 2013
• 作者: 中山幸輝;眞鍋一郎;砂河孝行;森岡勝樹;永井良三;小室一成
• 通讯作者: 小室一成