Isolation, Identification and Remedial Result of An Antibiotic Substance produced by Aphid Enterobacteria

一种蚜虫肠杆菌产生的抗生素物质的分离、鉴定及治疗效果

• 批准号: 01840028
• 负责人: ISHIKAWA Hajime
• 金额: $ 4.67万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01840028/
• 关键词: Aphida; Enterobacteria; Antibiotic substance; Anti-fungal substance; Honeydew; Hyphal growth; Oligopeptide; Secondary Endosymbiont; ウリ類ツル枯病菌; Staphylococcus

1. Gram-negative bacteria were collected from the honeydew excreted by the pea aphids, Acyrthosiphon pisum which had been reared in the laboratory for a long period. The bacteria were culture in LB and the medium was determined for its antibiotic activity after 20fold concentration by lyophilization. The result showed that the medium did not have antibiotic activity against either E.coli or Bacillus subtilis while the honeydew itself, even without concentration, inhibited the growth of E.coli very effectively.2. The cultured medium of the aphid enterobacteria was mixed with the potato dextrose medium for fungi, and determined for its anti-fungal activity in terms of hyphal growth retardation. As a result, it was shown that the aphid enterobacteria excreted a substance that inhibited the growth of several fungi specifically and effectively.3. The distribution experiments with some organic solvents suggested that this effective substance was an oligopeptide with molecular weight of about 1,000.4. There was no biochemical evidence that these enterobacteria were related to secondary endosymbionts that some aphid species harbor.

1.从实验室长期饲养的豌豆蚜(Acyrthosiphon Pisum)分泌的蜜露中分离到革兰氏阴性菌。将菌种接种于液体培养罐中，经20倍浓缩后进行冷冻干燥，测定其抗菌活性。结果表明，该培养基对大肠杆菌和枯草芽孢杆菌均无抗菌活性，而蜜露本身即使在没有浓度的情况下也能非常有效地抑制大肠杆菌的生长。将蚜虫肠杆菌的培养基配以马铃薯葡萄糖培养基作真菌，用菌丝生长抑制法测定其抗真菌活性。结果表明，蚜虫肠道细菌分泌出一种对几种真菌的生长有抑制作用的物质。在某些有机溶剂中的分布实验表明，该有效物质是一种相对分子质量约为1,000.4的寡肽。没有生化证据表明这些肠道细菌与一些蚜虫物种所含的次生内共生菌有关。

项目成果

Mizue Morioka: "Mutualism based on stress:Selective synthesis and phosphorylation of a stress protein by an intracellular symbiont." Journal of Biochemistry. (1992)

Mizue Morioka：“基于压力的互助：细胞内共生体选择性合成和磷酸化压力蛋白。”

Takema Fukatsu: "Synthesis and localization of symbionin,an aphid endosymbiont protein." Insect Biochemistry. (1992)

Takema Fukatsu：“共生素（一种蚜虫内共生蛋白）的合成和定位。”

Takema Fukatsu: "Synthesis and localization of symbionin,an aphid endosymbiont protein." Insect Biochemistry. (in press). (1992)

Takema Fukatsu：“共生素（一种蚜虫内共生蛋白）的合成和定位。”

O-Yu Kwon: "The longest 18S ribosomal RNA ever known:Nucleotide sequence and presumed secondary structure of the 18S rRNA of the pea aphid,Acyrthosiphon pisum." European Journal of Biochemistry. 202. 827-833 (1991)

O-Yu Kwon：“迄今为止已知的最长的 18S 核糖体 RNA：豌豆蚜 Acyrthosiphon pisum 的 18S rRNA 的核苷酸序列和推测的二级结构。”

Chiaki Ohtaka: "Structure of chaperonins from an intracellular symbiont and their functional expression in E.coli groE mutants." Journal of Bacteriology. (1992)

Chiaki Ohtaka：“细胞内共生体的伴侣蛋白结构及其在大肠杆菌 groE 突变体中的功能表达。”