Reseach on development of a drug from an endogeneous peptide that inhibits the platelet aggregation.

1.建立了高效液相色谱法测定蔬菜中S-（2-羧基丙基）谷胱甘肽的方法。利用该方法对多种蔬菜中的多肽进行了测定. S-（1，2-二羧乙基）谷胱甘肽（DCE-GS）对ADP或凝血酶诱导的血小板聚集有明显的抑制作用。但该肽对12-O-十四酰佛波醇-13-乙酸酯诱导的血小板聚集无抑制作用. DCE-GS合成酶是从大鼠肝胞浆中分离纯化的。该酶的分子量为53 kDa。谷胱甘肽和L-苹果酸的Km值分别为2.3和4.0 mM。4. DCE-GS单独使用对肝细胞DNA合成无影响。但与EGF联合使用时，能有效地促进EGF刺激的肝细胞DNA合成.再生肝DCE-GS含量升高，第2天达高峰，1周后恢复正常。

1. A high-performance liquid chromatographic determination of S-(2caroxypropyl)glutathione in vegetable was established. By this method the peptide was determined in various vegetables.2. S-(1, 2-Dicarboxyethyl)glutathione(DCE-GS)exerted a potent inhibition effect on ADP- or thrombin-induced platelet aggregation. The peptide, however, did not inhibit the platelet aggregation induced by 12-o-tetradecanoylphorbol-13-acetate.3. The DCE-GS-synthesizing enzyme was, purified from rat liver cytosolic fraction. The molecular mass of the enzyme was 53 kDa. The Km values for GSH and L-malate were 2.3 and 4.0 mM.4. DCE-GS alone had no effect on DNA synthesis of hepatocytes. However, when it was used with, - EGF, it effectively enhanced EGF-st'imulated DNA synthesis of hepatocytes.5. The DCE-GS level increased in regenerating rat liver, reaching a maximum level on the 2nd day and reverted to normal level in a week.