The physiological role of methylglyoxal pathway in mammal.

1.生物样品中的D-乳酸盐在一个小瓶反应中转化为强荧光物质。荧光物质通过具有荧光检测的HPLC分析。用这种方法可以测定500 fmol的D-乳酸。(J.色谱，566，（1991）18）2.观察运动后体液中D-乳酸、L-乳酸、丙酮酸和丙酮醛（MG）浓度的变化。运动后血浆D-乳酸、L-乳酸和丙酮酸水平升高。运动后红细胞MG含量明显降低。尿中D-乳酸和相关物质也进行了测定，但这些物质的水平显示与运动无关。EUR. J. Appl. Physiol. in press）3.用链脲佐菌素使大鼠患糖尿病或饥饿72 h。与对照组相比，糖尿病和饥饿大鼠血浆、肝脏和骨骼肌中D-乳酸水平显著升高。与此相反，在这些动物中，血浆、肝脏和骨骼肌中的丙酮酸水平显著下降。

1. D-Lactate in biological samples was converted into a strongly fluorescent substance in a one-vial reaction. The fluorescent substance was analysed by HPLC with fluorimetric detection. By this method 500 f mol of D-lactate could be determined. (J. Chromatogr., 566, (1991)18)2. The change in the concentrations of D-lactate, L-lactate, pyruvate and methylglyoxal(MG)in body fluids after exercise was investigated. Plasma levels of D-lactate, L-lactate and pyruvate increased after running. MG contents in red blood 'Cells decreased markedly after running. D-lactate and related substances were also determined in urine, but the level of these substances showed no relationship to exercise. Eur. J. Appl. Physiol. in press)3. Rats were made diabetic using streptozotocin or starved for 72 h. Diabetic and starved rats resulted in significant rise of D-lactate level in plasma, liver and skeletal muscle compared to the control group. In contrast with this, in these animals, pyruvate levels in plasma, liver and skeletal muscle fell markedly.