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Alternative to breast carcinogenicity test using a tissue culture method

Alternative to breast carcinogenicity test using a tissue culture method
使用组织培养方法替代乳腺癌致癌性测试
批准号:
01880013
负责人:
SUZUKI Kunio
金额:
$4.1万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Developmental Scientific Research
财政年份:
1989
资助国家:
日本
项目状态:
已结题
起止时间:
1989 至 1990

项目摘要

项目成果

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中文摘要
翻译
我们开发了一种新的体外微核试验,使用培养的小鼠乳腺上皮细胞作为组织特异性筛查可疑的乳腺癌致癌物。将妊娠小鼠乳腺上皮细胞培养于无血清的Ham’s F-12/Dulbecco’s改良Eagle培养基中,培养基中添加胰岛素、表皮生长因子、转铁蛋白、霍乱毒素、牛血清白蛋白和抗生素。在培养的第6天,将几种乳腺癌致癌物和其他化学物质添加到培养物中,并在处理后24小时对每1000个细胞的微核数量进行评分。在本研究中,五种乳腺癌致癌物n -甲基-n -亚硝基脲、n -丁基-n -亚硝基脲、7,12 -中二甲基苯(a)蒽、20-甲基胆蒽和2-乙酰氨基芴,以及一种直接致癌物n -甲基-n '-硝基-硝基胍增加了培养物中微核的发生率。由于所有的乳腺癌致癌物和一种直接致癌物在培养的小鼠乳腺上皮细胞中都以剂量相关的方式诱导微核,但其他致癌物通常不会,因此这些结果证明了使用微核作为短期、体外筛查可疑乳腺癌致癌物是合理的。
英文摘要
We developed a new in vitro micronucleus test using cultured mouse mammary epithelial cells as a tissue-specific screen for suspected breast carcinogens.Mammary epithelial cells from pregnant mice were cultured in serumfree Ham's F-12/Dulbecco's modified Eagle's medium supplemented with insulin, epidermal growth factor, transferin, cholera toxin, bovine serum albumin and antibiotics. Several breast carcinogens and other chemicals were added to the culturds at day 6 of culture, and the number of micronuclei per 1, 000 cells was scored at 24hr after treatment.In the present study, five breast carcinogens, N-methyl-N-nitrosourea, N-butyl-N-nitrosourea, 7, 12-mdimethylbenz (a) anthracene, 20-methylcholanthrene and 2-acetamidofluorene, and one direct carcinogen, N-methyl-N'-nitro-Nnitrosoguanidine increased the micronuclei incidence in the cultures.Since all breast carcinogens and a direct carcinogen induced micronuclei in the culture of mouse mammary epitherial cells in a dose related manner, but other agents generally do not, these results justify the use of micronuclei as a short term, in vitro screen of suspected breast carcinogens.
期刊论文(30)
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会议论文
Kunio Suzuki: "In vitro micronuclei in the mouse mammary epitherial cells grown under serumーfree culture conditions" Enviromental and Molecular Mutagenesis. 14. 195 (1989)
Kunio Suzuki:“在无血清培养条件下生长的小鼠乳腺上皮细胞的体外微核”环境和分子突变,14. 195 (1989)。
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通讯作者:
Kunio Suzuki: "In Vitro micronuclei in the mouse mammary epitherial cells ground under serum-free culture conditions" Environm. Mol. Mutagen. 14. 195 (1989)
Kunio Suzuki:“在无血清培养条件下研磨的小鼠乳腺上皮细胞中的体外微核”Environm。
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Kunio Suzuki and Nobue Shiragami: "In vitro micronucleus test for a screen of breast carcinogens" Mutation Research. (1991)
Kunio Suzuki 和 Nobue Shiragami:“用于筛查乳腺癌致癌物的体外微核试验”突变研究。
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发表时间:
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通讯作者:
Kunio Suzuki: "In vitro micronuclei in the mouse mammary epitherial cells grown under serum-free culture conditions" Environmental and Molecular Mutagenesis. 14. 195 (1989)
Kunio Suzuki:“在无血清培养条件下生长的小鼠乳腺上皮细胞的体外微核”环境和分子诱变。
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共 9 条
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    • 批准号:
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    • 项目类别:
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    • 负责人:
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    • 批准号:
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    • 项目类别:
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      $1.54万
    • 财政年份:
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    • 负责人:
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    • 批准号:
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    • 项目类别:
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    • 资助金额:
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    • 财政年份:
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    • 负责人:
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    • 依托单位:
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