Alternative to breast carcinogenicity test using a tissue culture method

使用组织培养方法替代乳腺癌致癌性测试

• 批准号: 01880013
• 负责人: SUZUKI Kunio
• 金额: $ 4.1万
• 依托单位: Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01880013/
• 关键词: Breast cancer; Carcinogens; Mammary epithelial cell; Micronucleus; Alternative; Mammary epitherial cell

We developed a new in vitro micronucleus test using cultured mouse mammary epithelial cells as a tissue-specific screen for suspected breast carcinogens.Mammary epithelial cells from pregnant mice were cultured in serumfree Ham's F-12/Dulbecco's modified Eagle's medium supplemented with insulin, epidermal growth factor, transferin, cholera toxin, bovine serum albumin and antibiotics. Several breast carcinogens and other chemicals were added to the culturds at day 6 of culture, and the number of micronuclei per 1, 000 cells was scored at 24hr after treatment.In the present study, five breast carcinogens, N-methyl-N-nitrosourea, N-butyl-N-nitrosourea, 7, 12-mdimethylbenz (a) anthracene, 20-methylcholanthrene and 2-acetamidofluorene, and one direct carcinogen, N-methyl-N'-nitro-Nnitrosoguanidine increased the micronuclei incidence in the cultures.Since all breast carcinogens and a direct carcinogen induced micronuclei in the culture of mouse mammary epitherial cells in a dose related manner, but other agents generally do not, these results justify the use of micronuclei as a short term, in vitro screen of suspected breast carcinogens.

本研究建立了一种新的体外微核试验方法，即用培养的小鼠乳腺上皮细胞进行组织特异性筛选。在培养的第6天加入几种乳腺癌致癌物和其它化学物质，在处理后24小时记录每1，000个细胞的微核数，本研究中，五种乳腺癌致癌物，N-甲基-N-亚硝基脲，N-丁基-N-亚硝基脲，7，12-二甲基苯并蒽，20-甲基胆蒽和2-乙酰氨基芴，和一种直接致癌物，N-甲基-N '-硝基-N-亚硝基胍可增加培养物中微核的发生率。由于所有乳腺致癌物和直接致癌物均以剂量相关的方式诱导小鼠乳腺上皮细胞培养物中的微核，但其他物质一般不诱导，因此这些结果证明微核可作为可疑乳腺致癌物的短期体外筛选方法。

项目成果

Kunio Suzuki: "In vitro micronuclei in the mouse mammary epitherial cells grown under serumーfree culture conditions" Enviromental and Molecular Mutagenesis. 14. 195 (1989)

Kunio Suzuki：“在无血清培养条件下生长的小鼠乳腺上皮细胞的体外微核”环境和分子突变，14. 195 (1989)。

Kunio Suzuki: "In Vitro micronuclei in the mouse mammary epitherial cells ground under serum-free culture conditions" Environm. Mol. Mutagen. 14. 195 (1989)

Kunio Suzuki：“在无血清培养条件下研磨的小鼠乳腺上皮细胞中的体外微核”Environm。

Kunio Suzuki and Nobue Shiragami: "In vitro micronucleus test for a screen of breast carcinogens" Mutation Research. (1991)

Kunio Suzuki 和 Nobue Shiragami：“用于筛查乳腺癌致癌物的体外微核试验”突变研究。

Kunio Suzuki: "In vitro micronuclei in the mouse mammary epitherial cells grown under serum-free culture conditions" Environmental and Molecular Mutagenesis. 14. 195 (1989)

Kunio Suzuki：“在无血清培养条件下生长的小鼠乳腺上皮细胞的体外微核”环境和分子诱变。

鈴木 邦夫: "動物の組織および細胞に特異的な発癌物質検出法" バイオインダストリ-. 7. 49-55 (1990)

Kunio Suzuki：“检测动物组织和细胞特异性致癌物的方法”Bioindustry 7. 49-55 (1990)。