Studies on antitumor effect for Head and Neck tumor

头颈肿瘤抗肿瘤作用研究

• 批准号: 04671244
• 负责人: SUZUKI Kunio
• 金额: $ 1.41万
• 依托单位: HINON UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04671244/
• 关键词: Colorectal cancer; Colon 26; Experimental model; Adjuvant chemotherapy; 温熱療法; 制癌剤; 扁平上皮癌; X線感受性; 温熱感受性; GSH量; ADF量; 温熱耐性; ヒートショック蛋白質; CHO細胞; 術前化学療法(CAF変法); 広背筋筋皮弁; 高LET線(中性子線); 生存率曲線; 増感効果; 頭頸部癌; Human Tumor Clonogenic Assey; colony 形

We report an experimental model of surgery with adjuvant chemotherapy by a footpad transplantation-tumor-transplanted limb amputation method using Colon 26, a tumor derived from mouse colorectal cancer. Colon 26 cells at an inoculum of 5*16^6 cells/mouse were transplanted into the footpads of 6-week-old male CDF_1 mice. In the untreated group, all mice died of cachexia within a short period of time, as seen in subcutaneous transplantation. In a preliminary experiment, tumor-transplanted limbs were amputated, resulting in an improved survival with the occurrence of lung metastasis. Next, the time of amputation suitable for an experimental model of surgery with adjuvant chemotherapy was estimated by the following experiment. Colon 26 cells at an inoculum of 5*10^6 cells/mouse adjusted to a volume of 0.025 ml were transplanted into the footpads of mice on Day 0.Six mice were used in each experimental group. The transplanted limbs were amputated from Day 1 to Day 6, and the mice were observed for 60 days. Autopsies were performed to determine the causes of death. The 60-day survival curves of the mice with amputation on Day 5 or Day 6 were closest to the clinical postoperative survival curves for colorectal cancer in humans. The results obtained suggest that this was a suitable experimental model for surgery with adjuvant chemotherapy. All deaths of these experimental mice were due to lung metastasis.

我们报道了一种实验性的手术模型，采用足垫移植-肿瘤移植截肢的方法，利用来源于小鼠结直肠癌的肿瘤-结肠26进行辅助化疗。将5×16^6个/只的结肠26细胞接种于6周龄雄性CDF_1小鼠的足垫内。在未经治疗的组中，所有的小鼠都在短时间内死于恶病质，就像皮下移植中看到的那样。在初步实验中，肿瘤移植的四肢被截肢，从而在发生肺转移的情况下提高了存活率。接下来，通过下面的实验估计适合于辅助化疗手术的实验模型的截肢时间。第0天将结肠26细胞接种于小鼠足垫内，每只小鼠接种5×10~(-6)个细胞，体积调整为0.025毫升。移植肢体于术后第1~6天截肢，观察60d。进行了尸检以确定死因。第5天或第6天截肢小鼠的60天生存曲线与人类结直肠癌的临床术后生存曲线最接近。结果表明，这是一种适合于手术辅助化疗的实验模型。所有这些实验小鼠的死亡都是由于肺转移。

项目成果

馬嶋秀行: "CHO細胞による温熱中性子線致死効果" 日大原子力研究所平成5年度報告書. (1994)

Hideyuki Mashima：“热中子束对 CHO 电池的致命效应”日本大学原子能研究所 1993 年报告（1994 年）。

笠倉 雄一: "術前化学療法が泰功し、広背筋M-Cflapにて一期的根治術が施行し得た乳癌の一例" 埼玉県医学会雑誌. 28.3. 23-26 (1993)

Yuichi Kasakura：“术前化疗成功并使用背阔肌 M-Cflap 进行一期根治的乳腺癌病例”，埼玉县医学会杂志 28.3（1993）。

Masasi FUJII,Mitugu KOCHI Kunio Suzuki, Takashi TANAKA: ""Experimental model of Surgery with Adjuvant chemotherapy for Colorectal Canser using Colon 26 Cells"-First report-" The Nihon univ. Journal of Medicin. Vol 36, No 5. 213-220 (1994)

Masasi FUJII、Mitugu KOCHI Kunio Suzuki、Takashi TANAKA：““使用结肠 26 细胞进行结直肠癌辅助化疗手术的实验模型”-第一份报告-”日本大学。

笠倉雄一: "術前化学療法が奏効し、広背筋MC-flapにて一期的根治術が施行し得た乳癌の一例" 埼玉県医学会雑誌. 28-3. 23-26 (1993)

Yuichi Kasakura：“术前化疗有效并使用背阔肌MC皮瓣进行一期根治的乳腺癌病例”，埼玉县医学会杂志28-3（1993年）。

馬嶋秀行: "細胞における温熱耐性の誘導とHSPの発現" 電子通信学会雑誌. (1994)

Hideyuki Mashima：“细胞耐热性的诱导和 HSP 的表达”电子与通信工程师学会杂志（1994 年）。