Using Drosophila eclosion to genetically dissect how central and peripheral clocks and the endocrine system interact to time behaviour

利用果蝇羽化从基因角度剖析中枢和外周时钟以及内分泌系统如何与时间行为相互作用

• 批准号: 398112454
• 负责人: Professor Dr. Christian Wegener
• 金额: --
• 依托单位: Lehrstuhl für Neurobiologie und Genetik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/398112454?language=en
• 关键词: Using; Drosophila; eclosion; genetically; dissect

For animals, it is vitally important to time and synchronise development and physiological activity of the different body systems and to adjust behaviour accordingly. The timing and synchronisation of different body systems and behaviour requires both a timer (represented by central and peripheral endogenous clocks) and an integrating communication system (represented by the (neuro)endocrine system). Yet, we know astonishingly little about the complex neuronal and endocrine pathways and underlying molecular and cellular signalling mechanisms by which endogenous clocks and neuroendocrine systems interact with each other. Such knowledge could provide a handle to understand and treat associated developmental disorders and circadian dysfunctions including impaired fertility, sleep disturbances and psychiatric problems that can result from long-term disruption of this integrated timing.The aim of this project is to dissect the interactions between developmental and circadian timers and the neuroendocrine system, and to start characterising cellular and molecular signalling principles underlying timed behaviour. A main focus here is on the interplay between peptide and steroid hormone signalling and its circadian control. We also aim to find out where in the brain circadian and developmental signals are integrated to time a specific behaviour.This specific behaviour will be the eclosion of the fruit fly Drosophila. The fruit fly appears a highly suited model system for this project as it is genetically and experimentally well tractable, and possesses a relatively low number of individually identifiable neurons. This in the long range offers the possibility to in completeness decipher neuronal and hormonal connections between central and peripheral clocks and target tissues from the molecular to the systemic level. Eclosion is well suited as it is timed by developmental and circadian timers, and its timing is under control of both peptide and steroid hormones. From a zoological point of view, understanding the architecture of the neuronal-endocrine network timing eclosion is of great interest since correct eclosion timing is most critical for the survival and fitness of insects and other arthropods, which represent the vast majority of animals on our planet.

对于动物来说，对不同身体系统的发育和生理活动进行计时和同步，并相应地调整行为至关重要。不同身体系统和行为的定时和同步需要定时器（由中央和外周内源性时钟表示）和整合通信系统（由（神经）内分泌系统表示）。然而，我们对复杂的神经和内分泌途径以及内源性时钟和神经内分泌系统相互作用的潜在分子和细胞信号传导机制知之甚少。这些知识可以提供一个手柄，以了解和治疗相关的发育障碍和昼夜节律功能障碍，包括生育能力受损，睡眠障碍和精神问题，可能会导致长期中断的这一综合times.This项目的目的是剖析发育和昼夜节律计时器和神经内分泌系统之间的相互作用，并开始描述定时行为背后的细胞和分子信号原理。这里的一个主要重点是肽和类固醇激素信号传导及其昼夜节律控制之间的相互作用。我们的目标也是找出在大脑中，昼夜节律和发育信号整合在一起，决定一个特定行为的时间，这个特定行为就是果蝇的羽化。果蝇似乎是一个非常适合这个项目的模型系统，因为它在遗传和实验上都很容易处理，并且具有相对较少的可单独识别的神经元。这在很长一段时间内提供了从分子水平到系统水平完全破译中枢和外周时钟与靶组织之间的神经元和激素连接的可能性。羽化是非常适合的，因为它是由发育和昼夜节律定时器定时，其时间是在肽和类固醇激素的控制下。 从动物学的角度来看，了解的神经内分泌网络的架构时序羽化是非常感兴趣的，因为正确的羽化时间是最关键的生存和健康的昆虫和其他节肢动物，这代表了我们这个星球上的绝大多数动物。