Effect of phytate degradation using phytase and soaking and resistant starch in barley on phosphorus utilization, gut health, and growth in pigs

使用植酸酶和浸泡抗性淀粉降解大麦中的植酸盐对猪的磷利用、肠道健康和生长的影响

基本信息

批准号：
398102117
负责人：
Dr. Charlotte Maria Elisabeth Heyer
金额：
--
依托单位：
Fachgebiet Tierernährung (460a)
依托单位国家：
德国
项目类别：
Research Fellowships
财政年份：
2018
资助国家：
德国
起止时间：
2017-12-31 至 2019-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/398102117?language=en
关键词：
Effect phytate degradation using phytase

项目摘要

The primary storage form of P in plant seeds is phytate (any salt of myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate); InsP6) that is poorly available for non-ruminant animals, such as the pig. Depending on activity of phytases and other phosphatases, InsP6 is hydrolyzed down to less phosphorylated inositol phosphates, such as myo-inositol, and available P. Feed processing techniques such as soaking and fermentation that promote activation of phytases to reduce InsP6 concentration in feed have gained in importance. Furthermore, InsP6 is an anti-nutritional factor in pig feed by chelating positively charged cations and affecting utilization of other dietary nutrients. Results on the interaction between P and the immune system are inconsistent, several studies in different species have shown a positive impact of dietary P and phytase supplementation on the adaptive immune response. Recent studies indicate that individual inositol phosphates or phosphate may also affect properties of the intestinal microbiota, including pathogenic microorganisms. The proposed study assesses effects of inositol phosphate degradation (using soaking, fermentation and microbial phytase supplementation) and resistant starch (low vs. high fermentable barley cultivars containing high and low amylose, respectively) in situ and in vivo using pigs on P utilisation, gut health and growth. The milestones of the proposed research project are: (1) Evaluation of the enzymatic degradation of phytate in situ using soaking and fermentation; (2) Effects of diets containing phytase supplementation and low vs. high fermentable barley cultivars soaked with lactic acid bacteria on nutrient digestibility, and (3) Impact of diets containing phytase supplementation and low vs. high fermentable barley cultivars soaked with lactic acid bacteria on growth performance in nursery pigs with special focus on the gastro-intestinal microbiota and immune system. The advantages of the planned research work are: (1) Results of the metabolism of InsP6 and lower inositol phosphates in vivo can be used target further increase InsP6 digestibility, to reduce supplementation with phosphate and to minimize P pollution from manure; (2) Using processing strategies (soaking and fermentation) and the supplementation of microbial phytase to the diet improves the understanding of phytase activity of different origins, and evaluates potential synergistic effects; (3) Description of the complex relationship between InsP6 in combination with starch using a common cereal, the gastro-intestinal microbiota and the immune system can be used to beneficially affect swine health and performance.

P在植物种子中的主要储存形式是植酸（肌醇盐的任何盐1,2,3,4,5,6-己高（二氢磷酸盐）； INSP6），对于非疾病的动物，例如猪，它们是不可用的。根据磷酸酶和其他磷酸酶的活性，INSP6被水解为较少的磷酸化肌醇磷酸盐，例如肌醇 - 肌醇和可用的P.饲料加工技术，例如浸泡和发酵，促进促进粉状酶的激活，以减少饲料中的INSP6浓度，从而在重要性上降低了INSP6浓度。此外，INSP6是通过螯合带正电的阳离子并影响其他饮食营养素的利用，是猪饲料中的抗营养因素。 P与免疫系统之间的相互作用的结果不一致，在不同物种中进行了一些研究表明，饮食P和补充植物酶对适应性免疫反应的积极影响。最近的研究表明，单个肌醇磷酸盐或磷酸盐也可能影响肠道菌群的性质，包括致病性微生物。拟议的研究评估了肌醇磷酸盐降解（使用浸泡，发酵和微生物植物酶补充剂）和耐药淀粉（分别为低和低淀粉糖的高和低淀粉液）的抗性淀粉的影响，并在体内使用PIPS在PITIVE，PITIVE，GUT健康和生长上进行体内和体内。拟议的研究项目的里程碑是：（1）使用浸泡和发酵评估原位植物的酶促降解；（2）含有含植物酶补充剂和低发酵性大麦品种的饮食对乳酸细菌浸泡的饮食对养分消化率的影响，以及（3）饮食中含有植酸酶补充剂的饮食和低发酵性的与高发酵性的大麦品种浸泡在乳酸系统中，乳酸细菌浸泡在疗养院中，并伴随着疗养院的良好型果皮，并在特殊的摄影中浸泡了果皮。计划研究工作的优点是：（1）可以使用INSP6和较低肌醇磷酸盐代谢的结果，可以使用目标进一步提高INSP6的消化率，以减少对磷酸盐的补充并最大程度地减少肥料中的P污染；（2）使用加工策略（浸泡和发酵）以及将微生物植物酶补充饮食可以提高对不同起源的植酸酶活性的理解，并评估潜在的协同作用；（3）使用普通谷物，胃肠道菌群和免疫系统的INSP6与淀粉结合使用的复杂关系可用于有益地影响猪的健康和性能。