Studies on the structure and function of bacterial 6S RNA, a putative transcriptional regulator

细菌 6S RNA（一种假定的转录调节因子）的结构和功能研究

• 批准号: 39851005
• 负责人: Professor Dr. Rolf Wagner
• 金额: --
• 依托单位: Institut für Physikalische Biologie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/39851005?language=en
• 关键词: Studies; structure; function; bacterial; 6S

6S RNA is a widespread non-coding RNA (ncRNA) in bacteria, which affects transcription. Due to the structural similarity with open promoter DNA 6S RNA binds to RNA polymerase and thereby inhibits the interaction with many but not all promoters. Based on the results of genome-wide transcriptome analysis we plan to investigate the molecular details of the still unresolved differences of the 6S RNA specificity, causing a differential regulation of individual genes. Moreover, under condition of 6S RNA depletion the significance of the strikingly changed expression level of the components constituting the translational apparatus shall be determined. The exceptionally striking property of RNA polymerase bound 6S RNA to direct the transcription of small de novo RNA products, thereby releasing the 6S RNA-mediated inhibition of RNA polymerase, is of special interest for the future investigation. To analyze the details of this process and to discover possible intrinsic functions of the de novo RNAs in the cell we plan to construct 6S RNA mutations and characterize their expression and function. In addition, experiments to identify potential 6S RNA-interacting molecules in the cell shall be performed. Finally, a screen for possible further functions of the interesting riboregulator, which may have escaped our notion so far, shall be carried out.

6S RNA是细菌中广泛存在的非编码RNA（ncRNA），影响转录。由于与开放启动子DNA的结构相似性，6S RNA与RNA聚合酶结合，从而抑制与许多但不是所有启动子的相互作用。基于全基因组转录组分析的结果，我们计划研究6S RNA特异性的仍未解决的差异的分子细节，导致单个基因的差异调节。此外，在6S RNA缺失的条件下，应确定构成翻译装置的组分的显著变化的表达水平的意义。RNA聚合酶结合6S RNA指导小的从头RNA产物的转录，从而释放6S RNA介导的RNA聚合酶抑制的异常惊人的性质是未来研究的特别兴趣。为了分析这一过程的细节并发现细胞中从头RNA可能的内在功能，我们计划构建6S RNA突变并表征其表达和功能。此外，还应进行实验以鉴定细胞中潜在的6S RNA相互作用分子。最后，应进行筛选，以寻找迄今为止可能没有我们概念的感兴趣的核糖核酸调节子的可能的进一步功能。