Studies of the function of membrane and soluble proteins and their biophysical properties.

研究膜和可溶性蛋白质的功能及其生物物理特性。

• 批准号: 10552333
• 负责人: Tobin R Sosnick
• 金额: $ 41.96万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-03-01 至 2028-02-29
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10552333
• 关键词: Address; Amides; Area; Bacteria; Biological; Biological Phenomena; Biological Sciences; Biology; Cells; Complex; Computing Methodologies; Deuterium; Disease; Exposure to; Hydrogen; Hydrogen Bonding; Individual; Innate Immune Response; Iron; Kinetics; Knowledge; Label; Learning; Mass Spectrum Analysis; Membrane; Membrane Proteins; Methods; Molecular Chaperones; Molecular Conformation; Nutrient; Pattern; Phase; Physical Function; Physical condensation; Physiologic pulse; Potassium Channel; Process; Property; Protein Chemistry; Protein Dynamics; Proteins; Protons; Research; Resolution; Sampling; Science; Stress; Structure; System; Techniques; Theft; biophysical chemistry; biophysical properties; computerized tools; deuteron; drug development; experience; improved; in vivo; interest; physical property; protein folding; skills; water channel

Over the past 50 years, the field of biophysical chemistry has learned an enormous amount about the relationships between the function, dynamics and folding of soluble proteins. The next stage in biological science involves addressing more complex problems on more challenging systems using increasingly sophisticated approaches augmented by computational methods. The Sosnick lab has followed this path. We are conducting studies of membrane proteins, condensates and disordered proteins. Our ability to address these topics arises in part from our expertise in the folding of soluble and more recently, membrane proteins. We build on this experience and knowledge to advance new or improve existing methods. The proposed research relies heavily on hydrogen exchange (HX), a method developed to study folding and dynamics yet possessing broad transferability to many methods. We plan to continue this approach and study the metabolite transport across a bilayer, stress-induced phase separation, and properties of disordered proteins in addition to membrane protein folding. Many of the projects are collaborative, leveraging our skills and interests with those of other labs, which further supports the value of our studies.

在过去的50年里，生物物理化学领域学到了巨大的 关于可溶性蛋白质的功能、动态和折叠之间的关系的数量。生物科学的下一阶段涉及使用越来越复杂的方法，加上计算方法，在更具挑战性的系统上解决更复杂的问题。索斯尼克实验室遵循了这条道路。我们正在对膜蛋白、凝集物和无序蛋白进行研究。我们能够解决这些问题的部分原因是我们在可溶性蛋白质折叠和最近的膜蛋白折叠方面的专业知识。我们以这些经验和知识为基础，推进新的或改进现有的方法。拟议的研究在很大程度上依赖于氢交换(HX)，这是一种研究折叠和动力学的方法，但具有广泛的可转换性。我们计划继续这种方法，并研究代谢物通过双层，应力诱导的相分离， 以及除了膜蛋白折叠之外的无序蛋白的性质。许多. 这些项目是协作的，利用我们与其他实验室的技能和兴趣， 这进一步支持了我们研究的价值。