Construction of versatile fluoroimmunoassay probe based on quench-release principle
Construction of versatile fluoroimmunoassay probe based on quench-release principle
批准号:
23656517
负责人:
UEDA Hiroshi
金额:
$2.33万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Challenging Exploratory Research
财政年份:
2011
资助国家:
日本
项目状态:
已结题
起止时间:
2011 至 2012
中文摘要
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英文摘要
With the aim of extending Quenchbody principle that can detect various antigens in a rapid and sensitive manner, an antibody binding protein probe that is fluorescence labeled at its terminus was constructed. When this probe was mixed with an anti-vimentin human Fab fragment, the dye of the probe was quenched by the Trp residues in the antibody. Moreover, when the antigen vimentin was added, dose-dependent release of the quenching was observed, thus resulted in successful detection of antigen by fluorescence increase.
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高親和性抗体結合タンパク質を利用した蛍光免疫センサー構築法の開発
开发使用高亲和力抗体结合蛋白的荧光免疫传感器构建方法
DOI:
--
发表时间:
2013
期刊:
影响因子:
--
作者:
[児島智樹, 大橋広行, 上田宏]
通讯作者:
上田宏
Quenchbodies : Antibody Probes That Fluoresce Upon Antigen Binding (Selected as oral presentation)
Quenchbodies:抗原结合时发出荧光的抗体探针(选择口头报告)
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[Hiroshi Ueda, Ryoji Abe, HiroakiTakagi, and Takahiro Hohsaka]
通讯作者:
and Takahiro Hohsaka
実験医学増刊―人と医学のステージへ拡大する細胞周期2013 中山敬一編
实验医学特别版-将细胞周期扩展到人类和医学阶段2013年编者:Keiichi Nakayama
DOI:
--
发表时间:
2013
期刊:
影响因子:
--
作者:
[鬼塚正義, 白井昭博, 大政健史, 吉川勝徳, 上田 宏]
通讯作者:
上田 宏
上田研究室
上田实验室
DOI:
--
发表时间:
期刊:
影响因子:
--
作者:
[]
通讯作者:
Demonstration of protein-fragment complementation assay using purified firefly luciferase fragments.
DOI:
10.1186/1472-6750-13-31
发表时间:
2013-03-28
期刊:
BMC biotechnology
影响因子:
3.5
作者:
[Ohmuro-Matsuyama Y, Chung CI, Ueda H]
通讯作者:
Ueda H
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