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Construction of versatile fluoroimmunoassay probe based on quench-release principle

Construction of versatile fluoroimmunoassay probe based on quench-release principle
基于猝灭释放原理的多功能荧光免疫探针的构建
批准号:
23656517
负责人:
UEDA Hiroshi
金额:
$2.33万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Challenging Exploratory Research
财政年份:
2011
资助国家:
日本
项目状态:
已结题
起止时间:
2011 至 2012

项目摘要

项目成果

UEDA Hiroshi的其他基金

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中文摘要
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英文摘要
With the aim of extending Quenchbody principle that can detect various antigens in a rapid and sensitive manner, an antibody binding protein probe that is fluorescence labeled at its terminus was constructed. When this probe was mixed with an anti-vimentin human Fab fragment, the dye of the probe was quenched by the Trp residues in the antibody. Moreover, when the antigen vimentin was added, dose-dependent release of the quenching was observed, thus resulted in successful detection of antigen by fluorescence increase.
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发表时间:
期刊:
影响因子: --
作者: []
通讯作者:
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