Development of Holographic Four-Dimensional Microscopy for Biomedical Diagnostics

研制了一种无透镜全息显微镜，用于水中游动微生物的四维测量。强度和相位图像具有高分辨率和大的深度，精确地重建在空气中，甚至在电介质中的运动物体。高分辨率显微层析成像也已开发用于通过使用无透镜反射型全息显微镜进行活体组织的光学测量。实验结果表明，层析三维成像与衍射极限分辨率。提出了一种新的无透镜超分辨全息显微术的研制方法。记录具有不同照明光入射角的四个或更多个全息图以生成具有大数值孔径（NA）值的一个全息图。所产生的全息图的NA可以被放大到大于1的值，并且显微镜的分辨率可以被提高到高于波长的一半的值。

A lens-less holographic microscope has been developed for 4-D measurement of microorganisms swimming in water. Intensity and phase images with a high resolving power and with a large depth are precisely reconstructed for moving objects in air or even in dielectric media. High-resolution microscopic tomography has also been developed for optical measurement of living tissue by using the lens-less reflection-type holographic microscope. Experimental results demonstrate tomographic 3-D imaging with diffraction limited resolution. A new method has been proposed in order to develop a super-resolution lens-less holographic microscopy. Four or more holograms with different incident angles of illuminating light are recorded to generate one hologram with a large value of the numerical aperture (NA). NA of the generated hologram can be enlarged up to a value larger than 1, and a resolution of the microscopy can be improved up to a value higher than one half of the wave length.