Development of ion-channel composed of nucleic acids

由核酸组成的离子通道的开发

• 批准号: 23657072
• 负责人: KATAHIRA Masato
• 金额: $ 2.5万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23657072/
• 关键词: 核酸; イオンチャネル; アプタマー; 4重鎖; プリオン; リボザイム; ４重鎖; リポソーム; 生物物理; コレステロール

The structure of a DNA/RNA quadruplex resembles that of an ion-channel. We tested if the quadruplex functions as an ion-channel. The quadruplex was embedded into liposome and a flow of ions was examined using fluorescent indicators. The results suggested that the quadruplex could function as an ion-channel. We also succeeded to elucidate the structural basis of the quadruplex as an anti-prion agent. The quadruplex was also utilized to switch the activity of a ribozyme.

DNA/RNA四链体的结构类似于离子通道。我们测试了四链体是否具有离子通道的功能。将四链体包埋到脂质体中，并使用荧光指示剂检查离子流。结果表明，四链体可能具有离子通道的功能。我们还成功地阐明了四链体作为抗朊病毒剂的结构基础。四链体也被用来转换核酶的活性。

项目成果

Intelligent ribozyme that switches its activity in response to K^+ through quadruplex formation

智能核酶，通过四链体形成响应 K^ 来切换其活性

• DOI: 10.1111/j.1742-4658.2012.08538.x
• 发表时间: 2012
• 期刊: FEBS Journal
• 影响因子: 5.4
• 作者: Nagata;T.;Sakurai;Y.;Hara;Y.;Mashima;T.;Kodaki;T.;Katahira;M.
• 通讯作者: M.

Creation of Novl Ribozyme whose Activity Switches on in Response to K+ via Quadruples Formation

新型核酶的创建，其活性通过四联体形成响应 K+ 而开启

• 发表时间: 2012
• 作者: Y.Yamaoki;T.Mashima;Y.Sakurai;Y.Hara;T.Kodaki;T.Nagata;M.Katahira
• 通讯作者: M.Katahira

Structure of Musashi1 in a complex with target RNA: the role of aromatic stacking interactions.

• DOI: 10.1093/nar/gkr1139
• 发表时间: 2012-04
• 期刊: Nucleic acids research
• 影响因子: 14.9
• 作者: Ohyama T;Nagata T;Tsuda K;Kobayashi N;Imai T;Okano H;Yamazaki T;Katahira M
• 通讯作者: Katahira M

Strctural analysisi of RNA aptamer in complex with prion protein, and its anti-prion activity

RNA适体与朊病毒蛋白复合物的结构分析及其抗朊病毒活性

• 发表时间: 2012
• 作者: T.Mashima;F,Nishikawa;Y.Kamatari;H.Fujiwara;T.Nagata;T.Kodaki;S.Nishikawa;K.Kuwata. M.Katahira
• 通讯作者: K.Kuwata. M.Katahira

Heat Shock Protein 70 Inhibits HIV-1 Vif-mediated Ubiquitination and Degradation of APOBEC3G*

• DOI: 10.1074/jbc.m110.166108
• 发表时间: 2011-01
• 期刊: The Journal of Biological Chemistry
• 作者: Ryuichi Sugiyama;H. Nishitsuji;A. Furukawa;M. Katahira;Y. Habu;H. Takeuchi;A. Ryo;H. Takaku