Development of temperature sensing fluorescent protein for in vivo thermal imaging induced by infrared laser irradiation to cells

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Single-cell gene induction is a powerful method for gene function and cell fate analysis. Infrared laser evoked gene operator method can enable this by utilizing heat shock response system to induce a target gene in the single cell of transgenic animals or plants which carries the target gene driven by heat shock promoter. However, because excess heating results in cell damage, we developed temperature-sensitive fluorescent protein and real time temperature sensing microscope system to improve the method