Critical analyses of "Sex" in homothallic Closterium peracerosum-strigosum-littorale complex

同宗新月藻-条纹藻-滨海复合体中“性”的批判性分析

• 批准号: 23657161
• 负责人: SEKIMOTO Hiroyuki
• 金额: $ 2.5万
• 依托单位: Japan Women's University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23657161/
• 关键词: ミカヅキモ; 有性生殖; ホモタリズム; ヘテロタリズム; 性分化; 性決定; ホモタリック

In Closterium peracerosum-strigosum-littorale complex, two types of conjugation produce zygotes; one is a conjugation between two complementary mating-type cells (mt+ and mt-) and the other is a conjugation between clonal cells. The former is referred to as heterothallism and the latter as homothallism.Heterothallic mating group II-B and homothallic strains (naga37) are phylogenetically closely related. The formation of hybrid zygotes between the homothallic cells and either heterothallic cells was confirmed. These results suggest that at least some of the homothallic gametangial cells possess the same characteristics as heterothallic mt- and/ or mt+ cells. On the other hand, progenies derived from the hybrid zygotes could not be obtained.Total RNA was collected from cells in several asexual and sexual reproductive stages and large scale RNAseq data was obtained by using new generation sequencer Hiseq2000. Finally, 132329 transcripts including many isoforms were established.

在过寒梭菌-异长梭菌-小梭菌复合体中，有两种接合方式产生合子；一种是两个互补的交配型细胞（mt+和mt-）之间的接合，另一种是克隆细胞之间的接合。前者称为异体性，后者称为同体性。异thalic交配组II-B和同thalic菌株（naga37）在系统发育上密切相关。证实了同thallic细胞与任何异thallic细胞之间形成杂交合子。这些结果表明，至少有一些同thallic配子体细胞具有与异thallic mt-和/或mt+细胞相同的特征。另一方面，从杂交合子衍生的后代不能得到。收集了多个无性生殖和有性生殖阶段细胞的总RNA，并使用新一代测序仪Hiseq2000获得了大规模的RNAseq数据。最后，共建立了132329个转录本，包括许多同工异构体。

项目成果

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• 发表时间: 2012
• 期刊: IFO Research communications
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• 通讯作者: 関本弘之

植物細胞リン酸輸送機構とその進化について

植物细胞磷酸盐转运机制及其演化

• 发表时间: 2015
• 作者: 藤原ひとみ;大西美輪;坂山英俊;石崎公庸;豊倉浩一;関本弘之;西山智明;七條千津子;小菅桂子;深城英弘;三村徹郎
• 通讯作者: 三村徹郎

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• 发表时间: 2012
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Identification of aquaporins from the unicellular charophycean alga, Closterium peracerosum-strigosum-littorale complex

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• 发表时间: 2011
• 作者: Abe;J.;Taki;K.;Nishiyama;T.;Tsuchikane;Y.;Sekimoto;H.
• 通讯作者: H.

Characterization of a conjugation processes in homothallic Closterium peracerosum-strigosum-littorale complex

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• 发表时间: 2013
• 作者: Tsuchikane;Y.;Sekimoto;H.
• 通讯作者: H.