Analyses of reproductive isolation of Closterium peracerosum-strigosum-littorale complex for the understanding of the speciation.

点击翻译按钮获取中文摘要

The Closterium peracerosum-strigosum-littorale complex (C. p-s-l. complex) is a unicellular alga, and is known to be composed of several reproductively isolated mating groups of heterothallic strains. We carried out field studies in foreign countries and in Japan, to isolate reproductively isolated C. p-s-l. complex. Finally, we established several unialgal clonal cultures from these fields.We studied phylogenetic relationship using DNA sequences of 1506 group I introns of these clones. Based on the alignment, significant phylogenetic relationships were observed between newly established mating group G and mating group I-E. Because genome project of cells belonging to the group I-E is ongoing, newly established group G would be suitable for the understanding of the mechanism of reproductive isolation, in the molecular biological aspect.