Analysis of function of endosymbiotic bacteria, Wolbachia, genes on the genome of Monochamus alternatus.

松墨天牛（Monochamus alternatus）是一种天牛，它是导致松树枯萎病的松材线虫的传播媒介。这种昆虫在常染色体上携带许多沃尔巴克氏体基因。本研究从昆虫的各个组织中提取RNA，进行转录组分析，以阐明Wolbachia基因表达的存在或不存在。 在每个组织中检测到几个似乎来自Wolbachia基因的阵列，阵列的数量在组织之间显著不同。这一结果表明，转移到松墨天牛中的Wolbachia基因中的一些基因已经在该昆虫中表达。

Monochamus alternatus is the longicorn beetle notorious as a vector of the pinewood nematode that causes the pine wilt disease. This insect caries many Wolbachia genes on an autosome. In this study, The RNA was extracted from each tissue of the insect, transcriptome analysis was conducted to clarify the presence or absence of the expression of Wolbachia genes. Several arrays that seem to be derived from Wolbachia genes in each tissue were detected, the number of the arrays was different between the tissues remarkably. This result suggests that some of the Wolbachia genes transfered to M.alternatus have expressed in the insect.