Development of technique to disrupt the reproductive function of Monochamus alternatus using an endosymbiotic bacterium, Wolbachia

沃尔巴克氏体是包括昆虫在内的节肢动物中的内共生细菌，能够通过引起细胞质不相容、孤雌生殖、雄性杀死或雌性化来操纵宿主繁殖。本研究研究了人工注射中国毛鼠沃尔巴克氏体（引起与寄主细胞质不亲和）对褐毛鼠的影响。注射沃尔巴克氏体个体与未注射沃尔巴克氏体个体的交配实验表明，只有注射沃尔巴克氏体个体与未注射沃尔巴克氏体个体交配产生的卵的孵化率显著降低。这说明中华月牙沃尔巴克氏体与交替田鼠存在细胞质不亲和性。

Wolbachia are endosymbiotic bacteria in arthropods including insects, and capable of manipulating host reproduction by causing cytoplasmic incompartibility, parthenogenesis, male killing or feminization. In this study, effect of artificial injection of the Wolbachia of Collosobruchus chinensis (cause cytoplasmic incompartibility to the host) to Monochamus alternatus was investigated. Mating experiments between Wolbachia-injected individuals and non-injected individuals revealed that only the hatching rate of eggs produced by mating of injected males x non-injected females decreased remarkably. This means that the Wolbachia of C. chinensis caused cytoplasmic incompartibility to M. alternatus.