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Genetic analysis of seeds for high sugar content suitable for production of bioethanol and high quality silage

适用于生产生物乙醇和优质青贮饲料的高糖种子的遗传分析

基本信息

批准号：
23658007
负责人：
SAZUKA Takashi
金额：
$ 2.41万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Challenging Exploratory Research
财政年份：
2011
资助国家：
日本
起止时间：
2011 至 2012
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23658007/
关键词：
ソルガム Sugary feterita 種子高糖性 starch debranching enzyme SU1 Sugary Feterita SUGARY1 エネルギー作物糖 sugary

项目摘要

Sugary feterita is a cultivar of Sorghum bicolor (L.) Moench, which accumulates sugar in the seeds. Here, we generated an F2 population by crossing Sugary Feterita with Nakei MS-3B which accumulates sugar in the seeds at low levels. An analysis of the segregation ratio of the F2 population indicated that the phenotype is determined by a recessive gene. A rough mapping analysis indicated that the corresponding gene is located on chromosome 7. Within the candidate region, a gene that encodes a starch biosynthetic enzyme, SbSU1, was located. This gene is an ortholog of maize SU1, the mutation of which is responsible for the high sugar content in the seeds of sweet corn. We carried out sequence analysis of the gene from both parental lines and found someSNPs. Two of them result in amino acid substitutions. After comparing SbSU1 with other orthologous genes from other species, we confirmed that Sugary Feterita possesses a mutant allele. SbSU1 transcripts increased toward flowering time, during which starch is being accumulated in wild-type (Feterita) seeds. These results suggest that the mutation(s) in SbSU1 of Sugary Feterita might also cause abnormal starch synthesis, consequently resulting in the accumulation of sugars in the seeds.

甜菜是高粱的一个栽培品种。莫恩奇，它在种子中积累糖分。在这里，我们通过将含糖的Feterita与Nakei MS-3B杂交产生F2群体，Nakei MS-3B在种子中低水平积累糖。对F_2群体分离比的分析表明，该群体的表型是由一个隐性基因决定的。粗略定位分析表明，该基因位于7号染色体上。在候选区域内，定位了一个编码淀粉生物合成酶SbSU1的基因。该基因是玉米SU1的同源基因，该基因的突变是甜玉米种子含糖量高的原因。我们对这两个亲本的基因进行了序列分析，发现了一些SNPs。其中两个会导致氨基酸替换。在将SbSU1与其他物种的其他同源基因进行比较后，我们证实了甜食Feterita具有突变的等位基因。SbSU1转录本在开花期间增加，在此期间，淀粉在野生型(Feterita)种子中积累。这些结果表明，含糖Feterita的SbSU1突变(S)也可能导致淀粉合成异常，从而导致种子中糖的积累。