Functional Analysis of auxin and microRNA in organ formation during rice embryogenesis

以水稻棒形胚1（cle 1）、棒形胚3（cle 3）和色氨酸缺乏矮秆1（tdd 1）3个突变体为材料，研究了生长素和miRNA对水稻胚胎发生器官分化的调控。两个突变体cle 1和cle 3的胚胎表型为棒状，tdd 1的胚胎表型为球状，这三个突变体在胚胎发生过程中的器官形成都有缺陷。定位克隆结果表明，cle 1和tdd 1的调控基因分别为OSDCL 1和ASB 1。详细的分析揭示了生长素和miRNA对水稻胚胎发生的重要调控作用。另外两个突变体，cre3和tdd1，也进行了分析。

The three rice mutants, club-shaped embryo1 (cle1), club-shaped embryo3 (cle3), tryptphan deficient dwarf1 (tdd1) were used to study the regulation for organ differentiation in rice embryogenesis by auxin and miRNA. The embryonic phenotype of two mutants, cle1 and cle3, are club-shaped, and that of tdd1 is globular ; the three mutants are defective for the organ formation during embryogenesis. The positional cloning indicates that the responsible genes for cle1 and tdd1 are OSDCL1 and ASB1, respectively. The detailed analysis revealed the important regulation by auxin and miRNA for rice embryogenesis. The other two mutants, cre3 and tdd1, were also analyzed.