Functional Analysis of auxin and microRNA in organ formation during rice embryogenesis

生长素和microRNA在水稻胚胎发生器官形成中的功能分析

• 批准号: 20570034
• 负责人: SAZUKA Takashi
• 金额: $ 3.08万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20570034/
• 关键词: イネ; 胚発生; 器官形成; オーキシン; マイクロRNA; 植物ホルモン; 生長生理; 国際情報交換

The three rice mutants, club-shaped embryo1 (cle1), club-shaped embryo3 (cle3), tryptphan deficient dwarf1 (tdd1) were used to study the regulation for organ differentiation in rice embryogenesis by auxin and miRNA. The embryonic phenotype of two mutants, cle1 and cle3, are club-shaped, and that of tdd1 is globular ; the three mutants are defective for the organ formation during embryogenesis. The positional cloning indicates that the responsible genes for cle1 and tdd1 are OSDCL1 and ASB1, respectively. The detailed analysis revealed the important regulation by auxin and miRNA for rice embryogenesis. The other two mutants, cre3 and tdd1, were also analyzed.

以水稻棒形胚1（cle 1）、棒形胚3（cle 3）和色氨酸缺乏矮秆1（tdd 1）3个突变体为材料，研究了生长素和miRNA对水稻胚胎发生器官分化的调控。两个突变体cle 1和cle 3的胚胎表型为棒状，tdd 1的胚胎表型为球状，这三个突变体在胚胎发生过程中的器官形成都有缺陷。定位克隆结果表明，cle 1和tdd 1的调控基因分别为OSDCL 1和ASB 1。详细的分析揭示了生长素和miRNA对水稻胚胎发生的重要调控作用。另外两个突变体，cre3和tdd1，也进行了分析。

项目成果

Mapping and Analysis of the CLUB-SHAPED EMBRYO1, CLE1.

俱乐部状胚胎 1、CLE1 的绘图和分析。

• 发表时间: 2008
• 期刊: Rice Gen.Newslett. 4
• 作者: Imamura;K.;Imai;H.;Nagato;Y.;Matsuoka;M.;Kitano;H.;Sazuka;T.
• 通讯作者: T.

A rice tryptophan deficient dwarf mutant, tdd1, contains a reduced level of indole acetic acid and develops abnormal flowers and organless embryos

• DOI: 10.1111/j.1365-313x.2009.03952.x
• 发表时间: 2009-10-01
• 期刊: PLANT JOURNAL
• 影响因子: 7.2
• 作者: Sazuka, Takashi;Kamiya, Noriko;Matsuoka, Makoto
• 通讯作者: Matsuoka, Makoto

Characterization and mapping of the CROWN ROOTLESS2 gene

CROWN ROOTLESS2 基因的表征和定位

• 发表时间: 2010
• 期刊: CRL2, in rice.Rice Gen.Newslett. 25
• 作者: Yamamoto;Y.;Inukai;Y.;Kitano;H.;Sazuka;T.;Matsuoka;M.
• 通讯作者: M.

Characterization and mapping of the CROWN ROOTLESS2 gene, CRL2, in rice.

水稻 CROWN ROOTLESS2 基因 CRL2 的表征和定位。

• 发表时间: 2010
• 期刊: Rice Gen.Newslet.
• 作者: Yamamoto;Y.;et al.
• 通讯作者: et al.

Mappring and characterization of the rice gene, CLUB-SHAPED EMBRY03, CLE3.

水稻基因 CLUB-SHAPED EMBRY03、CLE3 的定位和表征。

• 发表时间: 2010
• 期刊: Rice Gen.Newslet.
• 作者: Imamura;K.;et al.
• 通讯作者: et al.