Study on regulatory mechanisms of coloration of fruit of fruit trees by blue light

蓝光对果树果实着色的调控机制研究

• 批准号: 23658025
• 负责人: SUGAYA Sumiko
• 金额: $ 2.41万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23658025/
• 关键词: アントシアニン; ブドウ; 青色光; 果樹園芸学; 果実着色; 光

To investigate the effects of blue light illumination on fruit color development after verason of fruit development, anthocyanin accumulation and mechanisms of it were analyzed using 'Kyoho' (Vitis vinifera L. x Vitis labrusca L.) plants. Grape berries were illuminated by blue LED and the effect on peel color of grapes was investigated. The result indicated that blue light promotes anthocyanin synthesis through biosynthetic enzyme activities, especially, flavonoid 3'5' hydroxilase (F3'5'H). An irradiation experiments using in vitro grape and soil planted grape trees revealed that anthocyanin accumulation was induced by blue light irradiation. The upstream region of the F3'5'H gene contains several motif sequences for cis-transcriptional factors. These results suggest that blue light irradiation would be a way to improve coloration of grape peel.

以“巨峰”（葡萄属vinifera L.）为试材，研究了蓝光照射对果实发育过程中花色形成的影响。x葡萄属labrusca L.）植物采用蓝光LED对葡萄果实进行照明，研究其对葡萄果皮颜色的影响。结果表明，蓝光通过生物合成酶活性，尤其是类黄酮3'5'羟化酶（F3'5'H）的活性促进花色素苷的合成。利用离体葡萄和土培葡萄进行的辐照实验表明，蓝光辐照诱导了花青苷的积累。F3'5'H基因的上游区域含有几个顺式转录因子的基序序列。这些结果表明，蓝光照射可能是一种改善葡萄果皮着色的方法。