Isolation of citrus self-incompatibility gene based on the gene expression profile

基于基因表达谱分离柑橘自交不亲和基因

• 批准号: 23658033
• 负责人: KUBO TATSUYA
• 金额: $ 2.16万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23658033/
• 关键词: カンキツ; 自家不和合性

We performed gene expression subtraction of the difference in developmental stages of bud for the purpose of isolation of citrus self-incompatibility gene. As a result, citT209 was isolated by stylar specificity. Performing the function estimation, it was estimated be a Cu / Zn SOD. On the other hand, self-incompatibility of citrus was shown could be overcome by CuSO4 sprays to bud. In addition, it is enhanced by CuSO4 sprays to bud has revealed that the expression level of Cu / Zn SOD gene, which is an antioxidant enzyme. The pollen tube growth and germination of the pollen is inhibited by the active oxygen. Expression level of Cu / Zn SOD gene is increased by spraying CuSO4 to bud, as a result, was suggested that active oxygen is removed, overcoming the effects of incompatibility can be obtained.

为了分离柑橘自交不亲和基因，我们利用花蕾发育阶段的差异进行基因表达差减。结果表明，citT 209是通过花柱特异性分离得到的。经功能鉴定，该酶为Cu / Zn SOD。另一方面，用CuSO_4喷施柑桔芽，可以克服柑桔自交不亲和性。此外，CuSO 4喷雾对芽的促进作用揭示了抗氧化酶Cu / Zn SOD基因的表达水平。活性氧抑制花粉的萌发和花粉管的生长。用CuSO_4喷施花蕾可提高Cu / Zn SOD基因的表达量，从而清除活性氧，克服不亲和性的影响。