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Establishment of confined rat model "Netakiri" and molecular nutrition to recover from "Netakiri"

封闭大鼠模型“Netakiri”的建立和分子营养以从“Netakiri”中恢复

基本信息

批准号：
23658111
负责人：
ODA Hiroakiy
金额：
$ 2.5万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Challenging Exploratory Research
财政年份：
2011
资助国家：
日本
起止时间：
2011 至 2012
项目状态：
已结题

项目摘要

Bed rest confined condition, so called in Japanese “Netakiri” has been an important social issue in Japan. It is difficult that “Netakiri” people return to an independent lifestyle. Then we thought to look for food stuff to accelerate recovery from “Netakiri” condition. T o do these experiments, we have established confined rat model which is similar to “Netakiri” human. “Netakiri” rat model showed hypercholesterolemia and liver injury. The secretion rate of VLDL-cholesterol from liver was increased in “Natakiri” rats as compared to controls. The increased secretion of VLDL would explain the hypercholesterolemia. DNA microarray analysis indicated that genes related to cell cycles and apoptosis are changed. These result might explain the liver injury. Gene expression related to sulfur-containing amino acid metabolism was also changed. This change led to abnormal cholesterol or bile acid metabolism. The changed cholesterol and bile acid metabolism would lead hypercholesterolemia. Dietary egg white protein suppressed the hypercholesterolemia induced by “Netakiri” condition. And soy protein isolate suppressed both hypercholesterolemia and liver injury. These indicate that some dietary factors can ameliorate metabolic abnormality and liver injury induce by “Netakiri” condition.

卧床休息受限条件，在日本被称为“Netakiri”，在日本一直是一个重要的社会问题。内塔基里人回归独立生活方式困难重重。然后我们想寻找食物来加速从内塔基里病中恢复过来。为了进行这些实验，我们建立了类似于“Netakiri”人的受限大鼠模型。“Netakiri”大鼠模型表现为高胆固醇血症和肝损伤。“Natakiri”组大鼠肝脏极低密度脂蛋白-胆固醇的分泌率明显高于对照组。极低密度脂蛋白分泌增加可能是高胆固醇血症的原因。DNA微阵列分析表明，与细胞周期和细胞凋亡相关的基因发生了改变。这些结果可能解释了肝损伤的原因。与含硫氨基酸代谢相关的基因表达也发生改变。这种变化会导致胆固醇或胆汁酸代谢异常。胆固醇和胆汁酸代谢的改变会导致高胆固醇血症。日粮蛋清蛋白能抑制“Netakiri”状态所致的高胆固醇血症。大豆分离蛋白对高胆固醇血症和肝损伤均有抑制作用。提示某些饮食因素可改善“Netakiri”状态所致的代谢异常和肝损伤。