Isolation of taurine synthetic enzyme genes from fishes and mechanism of osmotic regulation

鱼类牛磺酸合成酶基因的分离及渗透调节机制

• 批准号: 23658176
• 负责人: SATOH Shuichi
• 金额: $ 2.5万
• 依托单位: Tokyo University of Marine Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23658176/
• 关键词: タウリン; 合成酵素; 魚類; 塩分

Cycteine sulfinic acid decarboxylase (CSD) genes are isolated from red sea bream and yellowtail. Expression analysis was also conducted in bastard halibut, red sea bream, seabass, and yellowtail. Total length of CSD was successfully isolated from red sea bream and yellowtail and deduced amino acid seqeuences from both species showed88.8 % homology and closely associated to CSD from Nile tilapia and platy, etc. Expressionof CSD was commonly observed in liver and pyloric caecae from different species.

半胱氨酸亚磺酸脱羧酶（CSD）基因是从红鲷和黄尾鱼中分离的。还在杂种大比目鱼、红鲷、鲈鱼和黄尾鱼中进行了表达分析。成功地从真鲷和黄尾鱼中分离到了CSD基因的全长，推导的氨基酸序列与尼罗罗非鱼、罗非鱼等的CSD基因同源性达88.8%，并与其他鱼类的CSD基因有密切的关联。

项目成果

海産魚類のタウリン合成酵素の単離および発現解析に関する研究

海水鱼类牛磺酸合酶的分离及表达分析研究

• 发表时间: 2013
• 作者: 芳賀穣;熊谷彩花;近藤秀裕;佐藤秀一.
• 通讯作者: 佐藤秀一.