In vivo diagnosisof viability for cell using water molecule structure analysis by aquaphotomics

为了利用近红外光谱中水分子结构随生物分子变化的吸收带来系统化关于水在生物和水体系中相互作用的丰富信息，利用近红外光谱这一新的科学领域，对与细胞活力有关的DNA进行了研究。利用水的OH伸缩振动的第一泛音(位于1300-1600 nm附近)分析了DNA光谱。制备了四种不同结构的DNA，并对其进行了近红外光谱分析。对每个DNA样本的DNA浓度进行定量，在1300-1600 nm范围内的每个回归载体上DNA样本之间出现差异。此外，对DNA的直线结构和环形结构的差异进行了分类，并对DNA结构分类的有效吸水带进行了研究。

DNA related to viability of cell was investigated by aquaphotomics which is new scientific area in order to systemize the abundant information concerning the interactions of water in biological and aqueous systems by the use of the absorption band of water molecule structure changing with biological molecules based on near infrared (NIR) spectroscopy. The first overtone of the OH stretching vibration of water (located around 1300 -1600 nm) was used for the analysis of DNA spectra. Four kinds of DNA which had different structure were prepared and measured by NIR spectroscopy. The DNA concentration was quantified for each DNA sample and the difference among DNA samples was appeared in each regression vector ranging 1300- 1600 nm. Moreover, the deference between straight and circular structure of DNA was classified and the effective water absorption bands for classification of DNA structure were investigated.