In vivo diagnosisof viability for cell using water molecule structure analysis by aquaphotomics

利用基于近红外光谱的水分子结构随生物分子变化的吸收波段，利用水分子组学这一新兴科学领域对与细胞活力相关的DNA进行了研究，以期将水在生物和水体系中相互作用的丰富信息系统化。水的OH拉伸振动的第一泛音（位于1300 -1600 nm左右）用于DNA光谱分析。制备了四种不同结构的DNA，并用近红外光谱对其进行了测定。每个DNA样本的DNA浓度被量化，在1300 ~ 1600 nm的回归向量上出现了DNA样本之间的差异。此外，还对DNA的直线结构和圆形结构进行了分类，并对DNA结构分类的有效吸水带进行了研究。

DNA related to viability of cell was investigated by aquaphotomics which is new scientific area in order to systemize the abundant information concerning the interactions of water in biological and aqueous systems by the use of the absorption band of water molecule structure changing with biological molecules based on near infrared (NIR) spectroscopy. The first overtone of the OH stretching vibration of water (located around 1300 -1600 nm) was used for the analysis of DNA spectra. Four kinds of DNA which had different structure were prepared and measured by NIR spectroscopy. The DNA concentration was quantified for each DNA sample and the difference among DNA samples was appeared in each regression vector ranging 1300- 1600 nm. Moreover, the deference between straight and circular structure of DNA was classified and the effective water absorption bands for classification of DNA structure were investigated.