In vivo diagnosisof viability for cell using water molecule structure analysis by aquaphotomics

利用水光组学分析水分子结构对细胞活力进行体内诊断

• 批准号: 23658211
• 负责人: ROUMIANA Tsenkova
• 金额: $ 1.91万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23658211/
• 关键词: アクアフォトミクス; 細胞; 組織; 分析化学; WAMACS; WAPS; 近赤外分光法; 細胞・組織

DNA related to viability of cell was investigated by aquaphotomics which is new scientific area in order to systemize the abundant information concerning the interactions of water in biological and aqueous systems by the use of the absorption band of water molecule structure changing with biological molecules based on near infrared (NIR) spectroscopy. The first overtone of the OH stretching vibration of water (located around 1300 -1600 nm) was used for the analysis of DNA spectra. Four kinds of DNA which had different structure were prepared and measured by NIR spectroscopy. The DNA concentration was quantified for each DNA sample and the difference among DNA samples was appeared in each regression vector ranging 1300- 1600 nm. Moreover, the deference between straight and circular structure of DNA was classified and the effective water absorption bands for classification of DNA structure were investigated.

利用近红外（NIR）光谱技术中水分子结构随生物分子变化的吸收带，将水组学这一新的科学领域应用于与细胞活力相关的DNA研究，以系统化生物和水溶液体系中水分子相互作用的丰富信息。水的OH伸缩振动的第一泛音（位于1300 - 1600 nm附近）用于DNA光谱的分析。制备了四种不同结构的DNA，并对其进行了近红外光谱分析。对每个DNA样品的DNA浓度进行定量，并且DNA样品之间的差异出现在1300- 1600 nm范围内的每个回归向量中。此外，还对DNA的直链和环状结构进行了分类，并对DNA结构分类的有效吸水谱带进行了研究。

项目成果

Spectral pattern of urinary water as a biomarker of estrus in the giant panda.

• DOI: 10.1038/srep00856
• 发表时间: 2012
• 期刊: SCIENTIFIC REPORTS
• 影响因子: 4.6
• 作者: Kinoshita, Kodzue;Miyazaki, Mari;Morita, Hiroyuki;Vassileva, Maria;Tang, Chunxiang;Li, Desheng;Ishikawa, Osamu;Kusunoki, Hiroshi;Tsenkova, Roumiana
• 通讯作者: Tsenkova, Roumiana

Aquaphotomics:How invisible Light Makes Water a Mirroron Molecular Level

Aquaphotomics：不可见光如何使水成为分子水平的镜像

• 发表时间: 2011
• 作者: Tsenkova;R.
• 通讯作者: R.

NIR spectroscopy: a tool for aquaphotomics

近红外光谱：水光光学工具

• 发表时间: 2011
• 作者: Tsenkova;R
• 通讯作者: R

Characterisation of hydrogen bond perturbations in aqueous systems using aquaphotomics and multivariate curve resolution-alternating least squares

• DOI: 10.1016/j.aca.2012.10.007
• 发表时间: 2013-01-08
• 期刊: ANALYTICA CHIMICA ACTA
• 影响因子: 6.2
• 作者: Gowen, A. A.;Amigo, J. M.;Tsenkova, R.
• 通讯作者: Tsenkova, R.

NIR Transmission spectra of saline solutions: a testing ground for Aquaphotomics

盐溶液的近红外透射光谱：水光组学的试验场

• 发表时间: 2011
• 作者: Gowen;A. (Tsenkova;R.)
• 通讯作者: R.)