Fundamental studies for development of a novel anti-tumor drug based on perchloric acid-soluble protein

点击翻译按钮获取中文摘要

Perchloric acid-soluble protein (PSP) has an anti-proliferating activity. Its activity seems to be suppressed in cells, because the PSP exists in proliferating cells. Crystal structural analysis of PSP shows that PSP exist as a homo-trimer. Our preliminary experiment, however, suggested PSP existed not only timer but also monomer or dimer. To elucidate that changing higher-order protein structure of PSP is related to cell proliferation, we evaluated the state of higher-order protein structure of PSP during liver regeneration after partial hepatectomy in rats. As a result, PSP existed as trimer in normal liver, but the ratio of trimer of PSP decreased after hepatectomy and the ration of monomer or dimer of PSP increased until liver weight returned to the intact weight. It is suspected monomer or dimer PSP not to suppress cell proliferation. Additionally, we identified the DNA position for expression and regulation of PSP mRNA. We identified two molecules to induce PSP expression.