Small non-coding RNAs in Streptomyces coelicolor

天蓝色链霉菌中的小非编码 RNA

• 批准号: 40095809
• 负责人: Professorin Dr. Beatrix Süß
• 金额: --
• 依托单位: Fachgebiet Molecular Genetics
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/40095809?language=en
• 关键词: Small; non; coding; RNAs; Streptomyces

Streptomyces coelicolor is a Gram-positive, GC-rich soil bacterium that serves as a model organism for the investigation of secondary metabolite production (antibiotics) and cell differentiation. Our research topic is the analysis of small regulatory RNAs (sRNAs) in this organism. During the last funding period we characterized the sRNA sc1. It controls the expression of the agarase DagA by a direct interaction with the ribosomal binding site of the target mRNA. Moreover, sc1 is involved in nitrogen metabolism and influences the production of antibiotics. We aim to characterize further targets of sc1 and unravel its role in the control of secondary metabolism. We have identified further ~ 800 potential sRNAs using a deep sequencing approach and selected a set of eight sRNAs for further analysis. They are differentially expressed in minimal media and show nitrogen or temperature dependence, respectively. We will perform a detailed structural and functional characterization of these candidates. Further on, we attempt to identify proteins involved in the sRNA-mRNA interactions since S. coelicolor lacks the major chaperon Hfq which facilitates this interaction in other organisms.

天蓝色链霉菌是一种革兰氏阳性、富含GC的土壤细菌，可作为研究次级代谢产物（抗生素）产生和细胞分化的模式生物。我们的研究主题是分析这种生物体中的小调控RNA（sRNA）。在上一个资助期间，我们表征了sRNA sc1。它通过与靶mRNA的核糖体结合位点直接相互作用来控制琼脂糖酶DagA的表达。此外，sc1参与氮代谢并影响抗生素的产生。我们的目标是进一步的sc1的特点和解开其在控制次生代谢的作用。我们使用深度测序方法进一步鉴定了约800种潜在的sRNA，并选择了一组8种sRNA进行进一步分析。它们在基本培养基中差异表达，并分别显示氮或温度依赖性。我们将对这些候选物进行详细的结构和功能表征。在此基础上，我们尝试从S.腔棘鱼缺乏在其它生物中促进这种相互作用的主要伴侣分子Hfq。