Diagnoses of animal diseases by monoclonal antibodies

1.肉毒中毒：制备抗肉毒梭菌提纯毒素lt、C_1和gt；及其轻链和重链的单抗。结果：1.利用单抗，将日本储存的所有肉毒杆菌毒素分成4组。小鼠肝炎(MH)：制备抗MH病毒分离株的单抗。部分单抗与病毒多聚体发生特异性反应，其余单抗与病毒核酸发生特异性反应。所有获得的单抗均与所有检测的MH病毒株发生交叉反应。马立克氏病(MD)：针对马立克氏病肿瘤建立的淋巴样细胞系的表面抗原而产生的单抗。这些单抗与MD鸡的肿瘤细胞和健康鸡的外周血细胞反应相同。在反应阳性鸡中，无论是肿瘤细胞还是血细胞中的阳性细胞率都较低。猪瘟(HC)：用猪白血病瘤细胞和猪瘟免疫猪的肿瘤细胞组成的杂交瘤细胞系统和其他一些系统建立杂交瘤细胞没有成功。然而，最近用丙型肝炎病毒感染猪的肾细胞免疫的小鼠的脾细胞可以获得单抗阳性细胞。流感和副粘病毒感染：用抗流感病毒HA或NA的单抗可以清楚地显示流感病毒的抗原改变和重组的来源。利用抗副粘病毒单抗证实了副粘病毒抗原表位的稳定性和抗原性的改变。牛白血病：针对地方性牛白血病(EBL)的肿瘤相关抗原而建立的单抗，能与所有临床白血病奶牛的肿瘤细胞发生特异性反应。这一发现表明，用单抗诊断EBL.7是可能的。感染性胰腺坏死：可产生部分单抗产生细胞。然而，由于细胞无法传代或细菌污染，细胞的维护失败。

1. Botulism: Monoclonal antibody (MAb) was produced against purified toxins <C_1> and D, their light and heavy chains of Clostridium botulinum. By the use of MAbs, toxins of all the C. botulinum, which were stored in Japan, were divided into 4 groups.2. Mouse hepatitis (MH): MAbs were prepared against an isolate of MH virus. Some of the MAbs specifically reacted with virus peplomer, and the remaining ones with virus nucleic acid. All the MAbs obtained cross-reacted with all the MH virus strains tested.3. Marek's disease (MD): MAbs were produced against surface antigens of a lymphoid cell line established from MD tumor. The MAbs reacted equally with tumor cell of chickens with MD and with peripheral blood cells of apparently healthy chickens. In reaction-positive chickens, ratio of positive-cell was relatively low both in the tumor and blood cells.4. Hog cholera (HC): Establishment of hybridoma was unsuccessful by the system consisted of tumor cell from pigs with leukosis and HC-immunized pigs, and by some other systems. However, MAb-positive cells have recently been available using spleen cell of mice immunized with kidney cells from HC-infected pig.5. Influenza and paramyxovirus infection: Antigenic alteration and the origion of a recombinant of influenza virus were clearly demonstrated by using MAbs against HA or NA of the virus. By the use of anti-paramyxovirus MAb, the stability of epitom and antigenic alteration of paramyxovirus were proved.6. Bovine leukosis: MAb established against tumor-associated antigen of enzootic bovine leukosis (EBL) specifically reacted with all the tumor cells obtained from cows with clinical leukosis. This finding suggested that the possibility to use the MAb for diagnosis of EBL.7. Infectious pancreatic necrosis: Some of MAb-producing cells were produced. However, maintenance of the cells were failed; because of inability of cell passage or bacterial contamination.