Diagnoses of animal diseases by monoclonal antibodies

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1. Botulism: Monoclonal antibody (MAb) was produced against purified toxins <C_1> and D, their light and heavy chains of Clostridium botulinum. By the use of MAbs, toxins of all the C. botulinum, which were stored in Japan, were divided into 4 groups.2. Mouse hepatitis (MH): MAbs were prepared against an isolate of MH virus. Some of the MAbs specifically reacted with virus peplomer, and the remaining ones with virus nucleic acid. All the MAbs obtained cross-reacted with all the MH virus strains tested.3. Marek's disease (MD): MAbs were produced against surface antigens of a lymphoid cell line established from MD tumor. The MAbs reacted equally with tumor cell of chickens with MD and with peripheral blood cells of apparently healthy chickens. In reaction-positive chickens, ratio of positive-cell was relatively low both in the tumor and blood cells.4. Hog cholera (HC): Establishment of hybridoma was unsuccessful by the system consisted of tumor cell from pigs with leukosis and HC-immunized pigs, and by some other systems. However, MAb-positive cells have recently been available using spleen cell of mice immunized with kidney cells from HC-infected pig.5. Influenza and paramyxovirus infection: Antigenic alteration and the origion of a recombinant of influenza virus were clearly demonstrated by using MAbs against HA or NA of the virus. By the use of anti-paramyxovirus MAb, the stability of epitom and antigenic alteration of paramyxovirus were proved.6. Bovine leukosis: MAb established against tumor-associated antigen of enzootic bovine leukosis (EBL) specifically reacted with all the tumor cells obtained from cows with clinical leukosis. This finding suggested that the possibility to use the MAb for diagnosis of EBL.7. Infectious pancreatic necrosis: Some of MAb-producing cells were produced. However, maintenance of the cells were failed; because of inability of cell passage or bacterial contamination.