DEVELOPMENT OF A ULTRAHIGH-VACUUM CHAMBERF TGO FACILITATE DEEP-ETCHING PREPARATION FOR BIOLOGICAL ELECTRON MICROSCOPY.

超高真空室 TGO 的开发有助于生物电子显微镜的深蚀刻制备。

• 批准号: 60870001
• 负责人: TONOSAKI Akira
• 金额: $ 8.32万
• 依托单位: YAMAGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60870001/
• 关键词: Clean vacuum; Deep-etching; Cryopump; Vacuum evaporation; 電顕試料; 凍結レプリカ法; 真空蒸着装置; 電顕試料作製法; フリーズ・エッチング法

SUB-CHAMBER HOUSING A DEEP-ETCHING STAGE AND PROVEDED WITH A CRYOPUMP, CRYO-U6 (ALVAC), WAS CONJUGATED WITH A UNIT OF VACUUM EVAPORATER. JFD-7000 (JEOL), WHICH HAD BEEN EARLIER DEVELOPED BY THE MEMBERS OF THE PRESENT PROJECT AND WAS CHARACTERIZED BY ITS TWO-WAY EVACUATION BY MEANS OF TWO SETS OF OIL-DIFFUSION PUMPS. MANY VACUUM-SPECIALISTS WERE SCEPTICAL OF SUCH A PLAN BECAUSE OF APPARENT DEMERIT OF HYBRIDIZING OF so-called clean and wet vacuum system. a vacuum of 10^<-9> ORDER WAS EVENTUALLY OBTAINED IN NUDE-GAUGE MONITORING MODE IN THE SUB-CHAMBER.THE SUB- AND MAIN CHAMBERS WERE ACTUALLY CONNECTED ONLY UPON USE BY A PASSAGE TGHROUGH WHICH A SPECIAMEN-HOLDER WAS PUSHED IN AND PULLED OUT. THE PASSAGE OTHERWISE STAYED CLOSED BY A SLIDE-VALVE TO KEEP THE VACUUM SYSTEM SEPARATED FROM EACH OTHER. BAKING HEATER AND METAL SEALS, BOTH REQUIREMENT FOR CLEAN-VACUUM MAINTAINANCE, WERE GIVEN UP BECAUSE THEY WERE SUPPOSED TO TAKE TOO MUCH SPACE OF DESIGNED SUB-CHAMBERS AND LOOKED UNREALISTIC FOR SUCH A CASUAL WORK AS FREEZE-ETCHING EXPERIMENTS, USED TO BE REPEATED INCESSANTLY EVERY TIME TO RUIN THE VACUUM.

容纳深蚀刻阶段并使用低温设备Cryo-U6（ALVAC）进行验证的子室与真空蒸发器装置结合。JFD-7000（JEOL），已由本项目的成员较早地开发，其特点是通过两套油膨胀阀进行双向排空。许多真空专家都对这种技术持谨慎态度，因为所谓的清洁和潮湿真空系统的混合具有明显的优势。最终<-9>在子室中的裸规监测模式下获得了10^量级的真空。只有在使用通道夹具时，才能实际连接主室和子室，并将样本夹持器推入和拉出。否则，通道通过滑动挡板保持关闭，以保持真空系统彼此分离。烘烤加热器和金属密封件，都是清洁真空维护的要求，被放弃了，因为它们被认为占用了太多的子室空间，而且对于冷冻蚀刻实验这样的偶然工作来说，看起来不现实，过去每次都要重复进行，以破坏真空。

项目成果

KIKUCHI TG, TONOSAKI A, TAKASAKA T: "DEVELOPMENT OF APICAL-SURFACE STRUCTURES OF MOUSE OTIC PLACODE." ACTA OTOLARYNGOL IN PRESS. (1988)

KIKUCHI TG、TONOSAKI A、TAKASAKA T：“小鼠耳板顶端表面结构的开发。”

Anatomical Record. 214:. 82-88 (1986)

解剖记录。

M.Ishikawa et al.: Cell Tiss.Res.249. 241-246 (1987)

M.Ishikawa 等人：Cell Tiss.Res.249。

A.Tonosaki et al. T.Imura,S.Maruse,T.Suzuki,eds.: In:Proc. XIth Int. Cong. Electron Microsc., Kyoto. III. 1848 (1986)

A.Tonosaki 等人。

Watanabe H.et al: Proc.【XI】 Int.Cong.Electr.Microsc. 3. 2487-2488 (1986)

Watanabe H.等：Proc.【XI】Int.Cong.Electr.Microsc. 3. 2487-2488 (1986)